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Gas-priming as a novel simple method of seed treatment with ethylene, hydrogen cyanide or nitric oxide
Acta Physiologiae Plantarum ( IF 2.6 ) Pub Date : 2021-07-17 , DOI: 10.1007/s11738-021-03288-0
Jan Kępczyński 1
Affiliation  

The gases used: ethylene (C2H4), hydrogen cyanide (HCN) and nitric oxide (NO) showed a high activity as inductors of germination in primary dormant or non-dormant seeds exposed to stress or suboptimal temperatures. So far, research on the role of ethylene, hydrogen cyanide and nitric oxide has involved these gases during seed germination. This work describes gas-priming as a novel method for treating air dry seeds of the genus Amaranthus serving as a model. Effects of C2H4, HCN or NO applied to dry seeds were similar to those obtained when the gases were used during seed germination for an identical period of time. Application of the gases to air dry seeds presents a new opportunity to study the role of those gases in germination of dormant and non-dormant seeds and to constrain effects of the gases from time 0. The prolonged treatment time for dry seeds (24 h) is convenient because if the treatment is started in the morning, there is sufficient time the next day for further experiments to begin, e.g. germination test in water or in solutions of other compounds. Moreover, it is important that the gas-treated seeds can be stored or transported prior to use. The gas-priming method allows to prepare treated seed samples which can be used in experiments either immediately or after storage in open air or in a closed container. Gas-priming with C2H4, HCN and NO is a simple and useful treatment of air-dried seeds, which opens up new useful possibilities for basic research on the role of those gases in releasing dormancy and seed germination of various plant species. In addition, the method may prove very useful in horticulture and agriculture in improving germination of gas-sensitive seeds.



中文翻译:

气体引发作为一种用乙烯、氰化氢或一氧化氮处理种子的新型简单方法

使用的气体:乙烯 (C 2 H 4 )、氰化氢 (HCN) 和一氧化氮 (NO) 作为暴露于压力或次优温度的初级休眠或非休眠种子的萌发诱导剂显示出高活性。迄今为止,关于乙烯、氰化氢和一氧化氮的作用的研究涉及这些气体在种子萌发过程中的作用。这项工作将气体引发描述为一种处理作为模型的属空气干燥种子的新方法。C 2 H 4 的影响、 HCN 或 NO 应用于干种子与在种子萌发过程中使用气体相同时间段获得的结果相似。将气体应用于风干种子为研究这些气体在休眠和非休眠种子发芽中的作用提供了新的机会,并从 0 时间开始限制气体的影响。 干燥种子的延长处理时间(24 小时)很方便,因为如果在早上开始处理,第二天就有足够的时间开始进一步的实验,例如在水中或其他化合物溶液中的发芽试验。此外,重要的是经过气体处理的种子可以在使用前储存或运输。气体引发方法允许制备处理过的种子样品,这些样品可以立即用于实验,也可以在露天或密闭容器中储存后用于实验。用 C 充气2 H 4、HCN 和NO 是一种简单而有用的风干种子处理方法,这为基础研究这些气体在释放各种植物物种的休眠和种子萌发中的作用开辟了新的有用的可能性。此外,该方法可证明在园艺和农业中在改善气敏种子发芽方面非常有用。

更新日期:2021-07-18
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