Pearl millet is an important C₄ cereal plant that possesses enormous capacity to survive under extreme climatic conditions. It serves as a major food source for people in arid and semiarid regions of south-east Asia and Africa. GRAS is an important transcription factor gene family of plant that play a critical role in regulating developmental processes, stress responses and phytohormonal signalling. In the present study, we have identified a total number of 57 GRAS members in pearl millet. Phylogenetic analysis clustered all the PgGRAS genes into eight groups (GroupI–GroupVIII). Motif analysis has shown that all the PgGRAS proteins had conserved GRAS domains and gene structure analysis revealed a high structural diversity among PgGRAS genes. Expression patterns of PgGRAS genes in different tissues (leaf, stem and root) and under various abiotic stress (drought, heat and salinity) were determined. Further, expression analysis was also carried out in response to various hormones (SA, MeJA, GA and ABA). The results provide a clear understanding of GRAS transcription factor family in pearl millet, and lay a good foundation for the functional characterisation of GRAS genes in pearl millet.

珍珠粟是一种重要的 C ₄谷类植物，在极端气候条件下具有巨大的生存能力。它是东南亚和非洲干旱和半干旱地区人们的主要食物来源。GRAS 是一个重要的植物转录因子基因家族，在调节发育过程、胁迫反应和植物激素信号传导方面发挥着关键作用。在本研究中，我们在珍珠粟中鉴定了总共 57 个 GRAS 成员。系统发育分析将所有PgGRAS基因分为八组（GroupI–GroupVIII）。基序分析表明所有 PgGRAS 蛋白都具有保守的 GRAS 结构域，基因结构分析显示PgGRAS之间具有高度的结构多样性基因。确定了PgGRAS基因在不同组织（叶、茎和根）和各种非生物胁迫（干旱、高温和盐度）下的表达模式。此外，还针对各种激素（SA、MeJA、GA 和 ABA）进行了表达分析。该结果为对珍珠粟中GRAS转录因子家族的了解提供了清晰的认识，为珍珠粟中GRAS基因的功能表征奠定了良好的基础。