Abstract

Biochar as a soil amendment, increases soil fertility and better agricultural production. In addition, the utilization of microbial bio-fertilizers improves soil health and reduces the necessity of excessive chemical fertilizer application. In this regard, the present research was conducted to evaluate the effect of bio-inoculants and bagasse biochar on root colonization, grain nutrients uptake, growth, and yield of barley, and biological activities in a calcareous soil. An experiment was conducted as a factorial arrangement on a completely randomized design with three replications in a greenhouse condition for seven months. The treatments consisted of six bio-fertilizer levels including control, Rhizophagus intraradices, Funneliformis mosseae, Micrococcus yunnanensis, Rhizophagus intraradices + Micrococcus yunnanensis, Funneliformis mosseae + Micrococcus yunnanensis, and three bagasse biochar levels of 0, 1%, and 2% w/w. The application of 2% biochar significantly increased root colonization (32.3%), grain nutrients uptake of N, P, Zn and Cu (10%, 10%, 10%, and 4.3%, respectively), biological yield (5.08%), soil microbial respiration (SMR) (11.3%), and microbial biomass C (MBS) (12.7%) compared to that of B₀. Individual application of bio-inoculants and their combination with biochar increased root colonization, grain nutrients (Fe, Zn, Cu, Mn, P, and N) uptake, biological and grain yield, 1000-kernel weight, SMR, and MBS. Application of plant growth promoting microorganisms and bagasse biochar simultaneously makes positive effects on barley and microbial activities, which is a promising strategy.

摘要

生物炭作为土壤改良剂，可提高土壤肥力和改善农业生产。此外，微生物生物肥料的利用改善了土壤健康，减少了过量施用化肥的必要性。在这方面，本研究旨在评估生物接种剂和甘蔗渣生物炭对大麦根系定植、谷物养分吸收、生长和产量以及钙质土壤中生物活性的影响。一项实验是在一个完全随机设计的因子安排上进行的，在温室条件下进行了三个重复，为期七个月。该疗法由六名生物肥料水平，包括控制，Rhizophagus intraradices，Funneliformis霉，微云南松, Rhizophagus intraradices + Micrococcus yunnanensis , Funneliformis mosseae + Micrococcus yunnanensis，以及三种甘蔗渣生物炭水平为 0、1% 和 2% w/w。施用 2% 生物炭显着提高根系定植率（32.3%），谷物养分吸收 N、P、Zn 和 Cu（分别为 10%、10%、10% 和 4.3%），生物产量（5.08%），与 B ₀相比，土壤微生物呼吸 (SMR) (11.3%) 和微生物生物量 C (MBS) (12.7%). 生物接种剂的单独应用及其与生物炭的组合增加了根系定植、谷物养分（Fe、Zn、Cu、Mn、P 和 N）的吸收、生物和谷物产量、千粒重、SMR 和 MBS。同时应用植物促生长微生物和甘蔗渣生物炭对大麦和微生物活性产生积极影响，这是一个很有前景的策略。