A novel synchronous acclimation strategy involving the continuous addition of sludge with chloramphenicol (CAP) was established to significantly enhance the formation of a highly efficient CAP-degrading anode biofilm in microbial fuel cells (MFCs). The highest power density of 414.00 mW/m² and CAP-tolerant concentration of 80 mg/L were obtained from the synchronous MFC, which were 2.05 and 1.67 times higher than those from the control MFC, respectively. The unique loose and porous biofilm with high permeability and cell viability supported by interwoven cobweb-shaped proteins facilitated mass and electron transfer, primarily leading to the improvements. Additionally, more bi-functional bacteria for electricity generation and CAP degradation (e.g., Pseudomonas and Enterococcus) were specifically selected, and more beneficial mutualism occurred among the microbes in the biofilm during the synchronous acclimation process. This study provides a possibility to improve the long-term operation efficiency of antibiotic-degrading electrode biofilms for bioelectrochemical technology through the use of a simple and efficient acclimation strategy.

建立了一种新的同步驯化策略，包括连续添加氯霉素（CAP）污泥，以显着增强微生物燃料电池（MFC）中高效 CAP 降解阳极生物膜的形成。同步 MFC 获得了 414.00 mW/m ²的最高功率密度和 80 mg/L 的 CAP 耐受浓度，分别是对照 MFC 的 2.05 和 1.67 倍。由交织的蛛网状蛋白质支持的具有高渗透性和细胞活力的独特松散多孔生物膜促进了质量和电子转移，主要导致了改进。此外，更多用于发电和 CAP 降解的双功能细菌（例如，假单胞菌和特异选择肠球菌，在同步驯化过程中，生物膜中的微生物之间发生了更多的有益共生。该研究为通过使用简单有效的驯化策略提高生物电化学技术中抗生素降解电极生物膜的长期运行效率提供了可能。