In this study, nitrobenzene was used as dummy template to synthesize a type of specific molecularly imprinted microspheres for chloramphenicol, and 4-nitroaniline was coupled with three fluorophores to synthesize three fluorescent tracers. Then a competitive fluorescence method was developed on a conventional microplate for detection of chloramphenicol in chicken and pork samples. This method contained only one sample-loading step, so one assay was finished within 30 min. The IC₅₀ was 1.8 ng/ml, and the limit of detection was 0.06 ng/g. The recoveries from chloramphenicol-fortified blank meat samples were in the range 67.5–96.2%. Furthermore, this method could be recycled three times. The detection results for some real meat samples were identical to that of a LC–MS/MS method. Therefore, this method could be used as a practical tool for routine screening for the residue of chloramphenicol in large number of meat samples.

中文翻译：

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分子印迹微球的合成及肉类中氯霉素的荧光检测方法的开发

本研究以硝基苯为虚拟模板合成了一类特定的氯霉素分子印迹微球，4-硝基苯胺与三种荧光团偶联合成了三种荧光示踪剂。然后在常规微孔板上开发了一种竞争性荧光方法，用于检测鸡肉和猪肉样品中的氯霉素。该方法仅包含一个上样步骤，因此在 30 分钟内完成了一项分析。IC ₅₀为 1.8 ng/ml，检出限为 0.06 ng/g。氯霉素强化空白肉样品的回收率在 67.5-96.2% 的范围内。此外，该方法可以循环使用3次。一些真实肉类样品的检测结果与 LC-MS/MS 方法的检测结果相同。因此，该方法可作为常规筛查大量肉类样品中氯霉素残留的实用工具。