Simón de Rojas Clemente y Rubio (1777 to 1827) is considered the father of modern ampelography. He developed the first scientific method for describing grapevine varieties through the examination of material he collected in Andalusia between 1802 and 1804. The Royal Botanical Garden in Madrid conserves the material he collected and preserved (leaves and shoots). This herbarium, the oldest of all grapevine variety herbaria, is invaluable for ampelographic and other studies (such as genetic diversity, molecular characterization, or viticultural history studies) and provides unique insight into early 19th century (the pre-phylloxera era) grapevine cultivation. The present work reports a DNA extraction protocol and a redefining of the simple sequence repeat primers routinely used in grapevine characterization, which allowed successful amplification of the ancient DNA extracted from Clemente’s herborized material.

中文翻译：

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215年前葡萄标本馆古代DNA的初步研究

Simón de Rojas Clemente y Rubio（1777 年至 1827 年）被认为是现代电流谱学之父。他通过检查 1802 年至 1804 年间在安达卢西亚收集的材料，开发了描述葡萄品种的第一个科学方法。马德里皇家植物园保存了他收集和保存的材料（叶子和枝条）。该标本馆是所有葡萄品种标本馆中最古老的标本馆，对于水培和其他研究（如遗传多样性、分子特征或葡萄栽培历史研究）非常宝贵，并提供了对 19 世纪早期（根瘤蚜前时代）葡萄栽培的独特见解。目前的工作报告了 DNA 提取方案和重新定义葡萄树表征中常规使用的简单序列重复引物，