Turmeric (Curcuma longa L. (Zingiberaceae)) is a rich source of medicinally important chemical compounds obtained from both pseudostem (aboveground part) and rhizome (underground part). However, the availability of disease-free elite varieties of the plant is highly restricted. Leaf spot caused by Colletotrichum curcumae Butler & Bisby and bacterial wilt caused by Ralstonia pseudosolanacearum Safni have been reported as major diseases responsible for reduced rhizome productivity in the turmeric plant. Meristem culture is a well-known alternative for production of disease-free mother rhizome. The aim of this investigation was to eradicate bacterial and fungal disease instances through culturing of meristematic tissue pretreated with 1.44 to −2.88 μM GA (gibberellic acid) followed by detection of disease contamination using PCR amplification and chemical assay. Meristem (0.5 to 2.0 mm) was identified as a good material for initiating tissue culture, as it exhibited 100% survival rate and absence of R. pseudosolanacearum and C. curcumae when tested with SPA (sucrose peptone agar), TZC (Kelman’s tripheny tetrazolium chloride), and PDA (potato dextrose agar) selective media. Plantlets derived from meristematic tissues of size 2.0 mm were healthy and displayed rapid recovery and uniformity in size. Disease-free status of plantlets (derived from meristem culture) was confirmed by PCR products of specific primers, RP1F/RP1R and RP3F/RP3R for R. pseudosolanacearum and CCactF/CCactR and CChisF/CChisR for C. curcumae. Moreover, shoot elongation using supplemented GA in the culture medium was successfully induced for meristem cutting. All meristem cultures pretreated with GA were confirmed to be contamination-free through TZC, SPA, and PDA media as well as PCR amplification. Based on this study, the 1.0 to 2.0 mm in size of meristem cutting and pretreatment with 1.44 to 2.88 μM GA for shoot elongation were successfully identified in addition to confirming the disease-free status of the plantlets using chemical assay and PCR products.

姜黄 ( Curcuma longa L. (Zingiberaceae)) 是从假茎（地上部分）和根茎（地下部分）中获得的具有重要药用价值的化合物的丰富来源。然而，该植物的无病优良品种的可用性受到高度限制。叶斑病引起炭疽病莪术巴特勒和Bisby引起的细菌性枯萎病青枯pseudosolanacearum据报道，Safni 是导致姜黄植物根茎生产力降低的主要疾病。分生组织培养是生产无病母根茎的一种众所周知的替代方法。本研究的目的是通过培养用 1.44 至 -2.88 μM GA（赤霉酸）预处理的分生组织，然后使用 PCR 扩增和化学分析检测疾病污染来根除细菌和真菌疾病实例。分生组织（0.5至2.0mm）被确定为良好的材料用于发起组织培养的，因为它显示出100％的存活率和不存在的R. pseudosolanacearum和C.莪术当使用 SPA（蔗糖蛋白胨琼脂）、TZC（凯尔曼三苯基氯化四唑）和 PDA（马铃薯葡萄糖琼脂）选择性培养基进行测试时。来自大小为 2.0 毫米的分生组织的小植株是健康的，并且显示出快速恢复和大小均匀。无病小植株的状态（来自分生组织培养衍生的）是通过特异性引物，RP1F / RP1R和RP3F / RP3R PCR产物确认R. pseudosolanacearum和CCactF / CCactR和CChisF / CChisR为C.莪术. 此外，在培养基中使用补充 GA 成功诱导枝条伸长以进行分生组织切割。通过 TZC、SPA 和 PDA 培养基以及 PCR 扩增，确认所有用 GA 预处理的分生组织培养物是无污染的。基于这项研究，除了使用化学分析和 PCR 产物确认小植株的无病状态外，还成功鉴定了 1.0 至 2.0 毫米大小的分生组织切割和用 1.44 至 2.88 μM GA 进行芽伸长的预处理。