Abstract—In mammals, chronic alcohol exposure disrupts the balance between excitatory and inhibitory neurotransmitter amino acids, including a decrease in GABAergic inhibitory function and an increase in glutamatergic excitatory activity. The effectiveness of the peptide anxiolytic selank against ethanol withdrawal anxiety and memory impairments was shown previously. In order to study the neurochemical mechanism of action of selank in an alcohol withdrawal model, we evaluated its effect on the content of neurotransmitter amino acids in various brain structures in rats. The experiments were performed with outbred male rats that consumed 10% ethanol solution as the only source of liquid for 30 weeks. During ethanol exposure, a subgroup of animals with a high level of motivation to consume alcohol was identified, and most of the work was carried out on these rats. Selank at an anxiolytic dose of 0.3 mg/kg was administered intraperitoneally for 7 days during ethanol withdrawal. Decapitation was performed 24 h after the last injection. In ex vivo experiments using HPLC/FD, selank was shown to prevent the ethanol withdrawal induced increase in the content of aspartic acid, glycine, and taurine in the hypothalamus, GABA in the n. accumbens, and aspartic acid and glycine in the striatum. These data indicate that during the alcohol deprivation period, the pharmacological effects of selank are associated with a decrease in the level of inhibitory neurotransmitter amino acids.

摘要—在哺乳动物中，长期接触酒精会破坏兴奋性和抑制性神经递质氨基酸之间的平衡，包括 GABA 能抑制功能的降低和谷氨酸能兴奋性活动的增加。肽抗焦虑 selank 对乙醇戒断焦虑和记忆障碍的有效性先前已显示。为了研究 selank 在酒精戒断模型中的神经化学作用机制，我们评估了其对大鼠各种脑结构中神经递质氨基酸含量的影响。实验是在远交雄性大鼠身上进行的，雄性大鼠消耗 10% 乙醇溶液作为唯一的液体来源，持续 30 周。在乙醇暴露期间，确定了一个具有高度饮酒动机的动物亚组，大部分工作都是在这些老鼠身上进行的。在乙醇戒断期间，以 0.3 mg/kg 的抗焦虑剂量腹膜内施用 Selank 7 天。在最后一次注射后 24 小时进行斩首。在使用 HPLC/FD 的体外实验中，selank 被证明可以防止乙醇戒断引起的下丘脑中天冬氨酸、甘氨酸和牛磺酸含量的增加，以及下丘脑中 GABA 的含量增加。n. 伏隔核，以及纹状体中的天冬氨酸和甘氨酸。这些数据表明，在酒精剥夺期间，selank 的药理作用与抑制性神经递质氨基酸水平的降低有关。