The mitochondrial genome of Neurospora crassa has been less studied than its nuclear counterpart, yet it holds great potential for understanding the diversity and evolution of this important fungus. Here we describe a new mitochondrial DNA (mtDNA) complete sequence of a N. crassa wild type strain. The genome with 64 839 bp revealed 21 protein-coding genes and several hypothetical open reading frames with no significant homology to any described gene. Five large repetitive regions were identified across the genome, including partial or complete genes. The largest repeated region holds a partial nd2 section that was also detected in Neurospora intermedia, suggesting a rearrangement that occurred before the N. crassa speciation. Interestingly, N. crassa has a palindrome adjacent to the partial nd2 repeated region possibly related to the genomic rearrangement, which is absent in N. intermedia. Finally, we compared the sequences of the three available N. crassa complete mtDNAs and found low levels of intraspecific variability. Most differences among strains were due to small indels in noncoding regions. The revisiting of the N. crassa mtDNA forms the basis for future studies on mitochondrial genome organization and variability.

中文翻译：

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重新审视粗糙脉孢菌线粒体基因组

粗糙脉孢菌的线粒体基因组比其核对应物的研究较少，但它在了解这种重要真菌的多样性和进化方面具有巨大潜力。在这里，我们描述了粗糙脉孢菌野生型菌株的新线粒体 DNA (mtDNA) 完整序列。具有 64 839 bp 的基因组揭示了 21 个蛋白质编码基因和几个假设的开放阅读框，与任何描述的基因没有显着的同源性。在整个基因组中鉴定了五个大的重复区域，包括部分或完整的基因。最大的重复区域包含在中间脉孢菌中也检测到的部分nd2部分，表明在粗糙脉孢菌之前发生了重排物种形成。有趣的是，粗糙脉孢菌在可能与基因组重排相关的部分nd2重复区域附近有一个回文，这在中间猪笼草中是不存在的。最后，我们比较了三种可用的粗糙脉孢菌完整 mtDNA的序列，发现种内变异水平较低。菌株之间的大多数差异是由于非编码区域中的小插入缺失。重新审视粗糙脉孢菌mtDNA 为未来研究线粒体基因组组织和变异性奠定了基础。