Cyclic AMP element modulator (CREM) is a transcription factor best known for its intricate involvement in spermatogenesis. The CREM gene encodes for multiple protein isoforms, which can enhance or repress transcription of target genes. Recent studies have identified fusion genes, with CREM as a partner gene in many neoplastic diseases. EWSR1-CREM fusion genes have been found in several mesenchymal tumors and in salivary gland carcinoma. These genes encode fusion proteins that include the C-terminal DNA-binding domain of CREM. We used a transcriptomic approach and immunohistochemistry to study the expression of CREM isoforms that include DNA-binding domains across human tissues. We found that CREM protein is widely expressed in almost all normal human tissues. A transcriptomic analysis of normal tissues and cancer showed that transcription of CREM can be altered in tumors, suggesting that also wild-type CREM may be involved in cancer biology. The wide expression of CREM protein in normal human tissues and cancer may limit the utility of immunohistochemistry for identification of tumors with CREM fusions:

环 AMP 元件调节剂 (CREM) 是一种转录因子，因其复杂地参与精子发生而闻名。的CREM基因编码多种蛋白质同种型，这可以提高或靶基因的抑制转录。最近的研究已经确定了融合基因，CREM作为许多肿瘤疾病的伙伴基因。EWSR1-CREM融合基因已在几种间充质肿瘤和唾液腺癌中发现。这些基因编码融合蛋白，包括 CREM 的 C 端 DNA 结合域。我们使用转录组学方法和免疫组织化学来研究 CREM 亚型的表达，其中包括人类组织中的 DNA 结合域。我们发现CREM蛋白在几乎所有正常人体组织中广泛表达。对正常组织和癌症的转录组学分析表明，CREM 的转录可以在肿瘤中发生改变，这表明野生型 CREM 也可能参与癌症生物学。CREM 蛋白在正常人体组织和癌症中的广泛表达可能会限制免疫组织化学用于鉴定具有CREM融合的肿瘤的效用：