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Rapid and cost-effective process based on insect larvae for scale-up production of SARS-COV-2 spike protein for serological COVID-19 testing
Biotechnology and Bioengineering ( IF 3.8 ) Pub Date : 2021-07-15 , DOI: 10.1002/bit.27889
Ignacio Smith 1, 2 , Gregorio Juan Mc Callum 1, 2 , Adriana Victoria Sabljic 3, 4 , Juan Ignacio Marfia 3, 4 , Silvina Sonia Bombicino 3, 4 , Aldana Trabucchi 3, 4 , Ruben Francisco Iacono 3, 4 , Joaquín Manuel Birenbaum 1, 2 , Susana Claudia Vazquez 1, 2 , Juan Mauricio Minoia 1, 2 , Osvaldo Cascone 2, 5 , María Gabriela López 6, 7 , Oscar Taboga 6, 7 , Alexandra Marisa Targovnik 1, 2 , Federico Javier Wolman 1, 2 , Matías Fingermann 5, 6 , Leonardo Gabriel Alonso 1, 2 , Silvina Noemí Valdez 3, 4 , María Victoria Miranda 1, 2
Affiliation  

Serology testing for COVID-19 is important in evaluating active immune response against SARS-CoV-2, studying the antibody kinetics, and monitoring reinfections with genetic variants and new virus strains, in particular, the duration of antibodies in virus-exposed individuals and vaccine-mediated immunity. In this study, recombinant S protein of SARS-CoV-2 was expressed in Rachiplusia nu, an important agronomic plague. One gram of insect larvae produces an amount of S protein sufficient for 150 determinations in the ELISA method herein developed. We established a rapid production process for SARS-CoV-2 S protein that showed immunoreactivity for anti-SARS-CoV-2 antibodies and was used as a single antigen for developing the ELISA method with high sensitivity (96.2%) and specificity (98.8%). Our findings provide an efficient and cost-effective platform for large-scale S protein production, and the scale-up is linear, thus avoiding the use of complex equipment like bioreactors.

中文翻译:

基于昆虫幼虫的快速且具有成本效益的方法,用于扩大生产用于血清学 COVID-19 检测的 SARS-COV-2 刺突蛋白

COVID-19 的血清学检测对于评估针对 SARS-CoV-2 的主动免疫反应、研究抗体动力学以及监测遗传变异和新病毒株的再感染情况非常重要,特别是病毒暴露个体和疫苗中抗体的持续时间介导的免疫。在这项研究中,SARS-CoV-2 的重组 S 蛋白在Rachiplusia nu中表达,一种重要的农艺瘟疫。一克昆虫幼虫产生的S蛋白量足以在本文开发的ELISA方法中进行150次测定。我们建立了一种 SARS-CoV-2 S 蛋白的快速生产工艺,该工艺对抗 SARS-CoV-2 抗体具有免疫反应性,并作为单一抗原用于开发具有高灵敏度 (96.2%) 和特异性 (98.8%) 的 ELISA 方法)。我们的研究结果为大规模 S 蛋白生产提供了一个高效且具有成本效益的平台,并且规模扩大是线性的,因此避免了使用生物反应器等复杂设备。
更新日期:2021-09-12
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