Abstract

Enantioanalysis of aspartic acid is of high importance for the metabolomics involved in gastric cancer. Therefore, two 3D stochastic sensors based on sulfur-doped graphene modified with protoporphyrin IX are reported for the enantioanalysis of aspartic acid. The 3D stochastic sensors were enantioselective with high sensitivity (the highest 10¹¹ and 10⁸ s⁻¹/mol L⁻¹ for l‐ and d-aspartic acid) and limits of quantification of 1 fmol L⁻¹ and 1 pmol L⁻¹ for l‐ and d-aspartic acids. The whole blood screening method based on utilization of the 3D stochastic sensors was validated using blood collected from healthy people and from patients with confirmed gastric cancer. Recoveries higher than 98.00% were recorded for enantioanalysis of aspartic acid in whole blood.

摘要

天冬氨酸的对映体分析对于涉及胃癌的代谢组学具有重要意义。因此，报道了两种基于用原卟啉 IX 改性的硫掺杂石墨烯的 3D 随机传感器，用于天冬氨酸的对映体分析。3D 随机传感器具有高灵敏度（对于l-和d-天冬氨酸最高为 10 ¹¹和 10 ⁸ s ^-1 /mol L ^{-1 ）和 1 fmol L -1}和 1 pmol L ^-1的定量限对于l - 和d-天冬氨酸。使用从健康人和确诊胃癌患者采集的血液验证了基于 3D 随机传感器的全血筛查方法。全血中天冬氨酸的对映体分析记录了高于 98.00% 的回收率。