Previous studies have shown MALDI-TOF MS to be a powerful tool in wine yeast identification and potential prediction of application. However, it is also established that substrate composition influences protein expression, but the degree to which this may affect MALDI-TOF spectra (and analytical results thereof) has not been fully explored. To further inform assay optimisation, the influence on MALDI-TOF spectra was determined using eight Saccharomyces strains of diverse origins cultivated on grape juices from Pinot Noir and Chardonnay varieties, synthetic grape juice, and laboratory-grade artificial culture media (YPD broth and agar). Our results demonstrated significant influences of culture media on strain MALDI-TOF spectra. Yeast culture on YPD agar is recommended for taxonomic studies, with YPD broth culture of S. cerevisiae offering improved intra-subspecific differentiation Furthermore, our data supported a correlation between MALDI spectra and the potential industrial application of individual yeast strains.

先前的研究表明，MALDI-TOF MS 是葡萄酒酵母鉴定和潜在应用预测的有力工具。然而，还确定底物组成影响蛋白质表达，但是这可能影响 MALDI-TOF 光谱（及其分析结果）的程度尚未得到充分探索。为了进一步优化分析，使用八种酵母菌确定了对 MALDI-TOF 光谱的影响在来自黑比诺和霞多丽品种的葡萄汁、合成葡萄汁和实验室级人工培养基（YPD 肉汤和琼脂）上培养的不同来源的菌株。我们的结果证明了培养基对菌株 MALDI-TOF 光谱的显着影响。建议将 YPD 琼脂上的酵母培养用于分类学研究，酿酒酵母的YPD 肉汤培养提供改进的亚种内分化此外，我们的数据支持 MALDI 谱与单个酵母菌株的潜在工业应用之间的相关性。