Thermoprotective carrier agents increase bacterial viability during the drying process. We investigated the ability of various carriers, including skim milk powder, casein, and whey protein, to improve the survival rate of Lactobacillus brevis RK03 under gastrointestinal tract conditions. Employing optimized top fluid bed drying (TFBD) conditions (40 min at 50 °C), the highest bacterial survival rate (95 %) was achieved using casein and whey protein as carriers for RK03. Additionally, whey protein-microencapsulated RK03 displayed the highest survival rates in gastric juice at pH 2.0 (96 %) and 3.0 (75 %). Further, whey protein-microencapsulated RK03 exhibited better dispersibility and solubility than RK03 microencapsulated with other carriers, displayed a slower degradation rate at 50 °C and 60 °C, and had the lowest activation energy (30.27 J/mol). These results indicate that the use of whey protein as a carrier during TFBD is suitable for the production of RK03 probiotic powder.

热保护载体剂可在干燥过程中提高细菌活力。我们研究了各种载体，包括脱脂奶粉、酪蛋白和乳清蛋白，提高短乳杆菌存活率的能力RK03 在胃肠道条件下。采用优化的顶部流化床干燥 (TFBD) 条件（50 °C 下 40 分钟），使用酪蛋白和乳清蛋白作为 RK03 的载体实现了最高的细菌存活率（95%）。此外，乳清蛋白微囊化 RK03 在 pH 2.0 (96 %) 和 3.0 (75 %) 的胃液中显示出最高的存活率。此外，乳清蛋白微胶囊化的 RK03 表现出比其他载体微胶囊化的 RK03 更好的分散性和溶解性，在 50°C 和 60°C 下显示出更慢的降解速率，并且具有最低的活化能（30.27 J/mol）。这些结果表明在TFBD过程中使用乳清蛋白作为载体适合生产RK03益生菌粉。