当前位置: X-MOL 学术J. Porphyr. Phthalocyanines › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Tuning the morphology of mesoscopic structures of porphyrin macrocycles functionalized by an antimicrobial peptide
Journal of Porphyrins and Phthalocyanines ( IF 1.5 ) Pub Date : 2020-05-20 , DOI: 10.1142/s1088424619502006
Rita Cimino 1 , Elisa Grelloni 1 , Gabriele Magna 1 , Donato Monti 1 , Manuela Stefanelli 1 , Emanuela Gatto 1 , Ernesto Placidi 2 , Francesca Biscaglia 3 , Marina Gobbo 3 , Mariano Venanzi 1
Affiliation  

The aggregation properties of two peptide–porphyrin conjugates were investigated by optical spectroscopy and microscopy imaging with nanometer resolution. Specifically, a tetraphenylporphyrin platform was functionalized by (L)-magainin, a 23-residue long antimicrobial peptide, and by a (L)-magainin analogue differing from the parent peptide by a single residue substitution, i.e. an Ala vs. Phe replacement in the position 5 of the peptide chain. Spectroscopic and microscopy results show that this single-site substitution has a small effect on the secondary structure attained by the two peptide analogues, but deeply affects the morphology of the mesoscopic structures deposited on hydrophilic mica from methanol/water solutions. In particular, only the Ala-substituted peptide-porphyrin conjugate was shown to be able to form micrometric fibrils, coating homogeneously a hydrophilic mica surface. These results pave the way for potential applications of porphyrin-peptide compounds in localized photodynamic therapy and for designing solid-state stereoselective sensors.

中文翻译:

调节由抗菌肽功能化的卟啉大环介观结构的形态

通过具有纳米分辨率的光学光谱和显微镜成像研究了两种肽-卟啉缀合物的聚集特性。具体来说,四苯基卟啉平台由 (L)-magainin(一种 23 个残基长的抗菌肽)和通过单个残基取代而与亲本肽不同的 (L)-magainin 类似物进行功能化,一世.e.阿拉对比肽链第 5 位的 Phe 置换。光谱和显微镜结果表明,这种单点取代对两种肽类似物所获得的二级结构影响很小,但对甲醇/水溶液中亲水性云母上沉积的介观结构的形态有很大影响。特别是,只有 Ala 取代的肽-卟啉共轭物被证明能够形成微米原纤维,均匀地包覆亲水云母表面。这些结果为卟啉肽化合物在局部光动力治疗和设计固态立体选择性传感器中的潜在应用铺平了道路。
更新日期:2020-05-20
down
wechat
bug