Zearalenone (ZEN), one of the most dangerous mycotoxins, causes enormous economic losses in the food and feed industries. To solve the problem of ZEN pollution, ZEN detoxifying enzymes are in emergent need. In this study, a zearalenone lactonohydrolase from Trichoderma aggressivum, denoted as ZHD-P, was heterologously expressed and characterized. The intracellular ZHD-P from E. coli BL21(DE3) exhibited high activity for ZEN degradation (191.94 U/mg), with the optimal temperature and pH of 45 °C and 7.5–9.0, respectively. With excellent temperature stability, the intracellular ZHD-P retained 100% activity when it was incubated at 25–40 °C for 1 h. Furthermore, we firstly constructed an E. coli cell surface display system for ZHD-P. The surface-displayed ZHD-P exhibited high activity against ZEN and showed optimal activity at 40 °C and pH 9.0. With superior pH stability, the surface-displayed ZHD-P retained 80% activity when it was incubated at pH 5.0–11.0 for 12 h. Interestingly, the metal ions tolerance of the surface-displayed ZHD-P was better than the intracellular form. Additionally, the surface-displayed ZHD-P could be reused four times with the residual enzyme activity of more than 50%. The biotoxicity assessment using P. phosphoreum T3 indicated that ZEN could be degraded into hypotoxic products by the intracellular or surface-displayed ZHD-P. ZHD-P could be feasible for ZEN detoxification.

玉米赤霉烯酮 (ZEN) 是最危险的霉菌毒素之一，在食品和饲料行业造成巨大的经济损失。为解决ZEN污染问题，急需ZEN解毒酵素。在这项研究中，来自Trichoderma agressivum 的玉米赤霉烯酮内酯水解酶，称为 ZHD-P，被异源表达和表征。来自大肠杆菌BL21（DE3）的细胞内 ZHD-P对 ZEN 降解表现出高活性（191.94 U/mg），最适温度和 pH 值分别为 45 °C 和 7.5-9.0。细胞内 ZHD-P 具有出色的温度稳定性，在 25-40°C 孵育 1 小时后仍保持 100% 的活性。此外，我们首先构建了一个大肠杆菌ZHD-P的细胞表面显示系统。表面展示的 ZHD-P 对 ZEN 表现出高活性，并在 40 °C 和 pH 9.0 时表现出最佳活性。由于具有优异的 pH 稳定性，表面展示的 ZHD-P 在 pH 5.0-11.0 下孵育 12 小时时保留了 80% 的活性。有趣的是，表面显示的 ZHD-P 的金属离子耐受性优于细胞内形式。此外，表面展示的 ZHD-P 可重复使用四次，残留酶活性超过 50%。使用 P.phosphoeum T3 进行的生物毒性评估表明，ZEN 可以通过细胞内或表面展示的 ZHD-P 降解为低毒性产物。ZHD-P 可用于 ZEN 解毒。