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Metabolite changes of apple Penicillium expansum infection based on a UPLC-Q-TOF metabonomics approach
Postharvest Biology and Technology ( IF 7 ) Pub Date : 2021-07-14 , DOI: 10.1016/j.postharvbio.2021.111646
Youming Shen 1, 2, 3 , Mingyu Liu 1, 2, 3 , Jiyun Nie 4 , Ning Ma 5 , Guofeng Xu 1, 2, 3 , Jianyi Zhang 1, 2, 3 , Yinping Li 1, 2, 3 , Haifei Li 1, 2, 3 , Lixue Kuang 1, 2, 3 , Zhiyuan Li 6
Affiliation  

The Penicillium expansum (P. expansum) infection causes apple postharvest deterioration and declines the quality of apple derived products. Investigation into the metabolic process of apple P. expansum infection will help to illustrate the mechanism of pathology and discover the biomarkers for quality control. Here, the changes of metabolite during the apple P. expansum infection were explored by ultra performance liquid chromatography and the quadrupole-time of flight mass spectrometry (UPLC-Q-TOF/MS) based metabonomics. Between four group of 32 samples, average 1720 positive and 1761 negative peaks were obtained, which aligned into comparable peaks of 5048 and 4945. Significantly metabonomic differences were observed between the control and P. expansum infected samples. A total of 485 differential metabolites were screened. In these different metabolites, the majority of sugars were decreased during P. expansum infection, whereas the majority of organic acids, amino acids and peptides were increased. Changes in the profile of phenolics and fatty acids were diverse, and major of them were increased for disease resistance. Disturbed metabolic pathways in early infection were mainly secondary metabolite biosynthesis, ATP-binding cassette transporters, amino acid and carbon metabolism. The intergroup comparison obtained 140 differential metabolites to represent the core metabolic changes during infection. And 27 metabolites were strongly increased along with the disease process, which could aid the biomarker discovery. This study highlights the differential metabolites during the apple P. expansum infection, and provide a basis for further investigation.



中文翻译:

基于 UPLC-Q-TOF 代谢组学方法的苹果膨胀青霉感染代谢物变化

扩展青霉扩展青霉)感染引起苹果收获后恶化和下降的苹果衍生产品的质量。调查苹果P. expansum感染的代谢过程将有助于阐明病理机制并发现质量控制的生物标志物。这里,苹果P.expansum期间代谢物的变化通过超高效液相色谱和基于四极杆飞行时间质谱 (UPLC-Q-TOF/MS) 的代谢组学研究感染。在四组 32 个样本之间,获得了平均 1720 个阳性峰和 1761 个阴性峰,它们与 5048 和 4945 个可比较的峰对齐。在对照和P. expansum感染的样品之间观察到显着的代谢组学差异。共筛选了 485 种不同的代谢物。在这些不同的代谢物中,大部分糖在P. expansum期间减少感染,而大多数有机酸、氨基酸和肽增加。酚类和脂肪酸的变化是多种多样的,其中大部分增加了抗病性。早期感染中受干扰的代谢途径主要是次级代谢物生物合成、ATP 结合盒转运蛋白、氨基酸和碳代谢。组间比较获得了 140 种不同的代谢物,以代表感染期间的核心代谢变化。27 种代谢物随着疾病的进程而强烈增加,这有助于生物标志物的发现。本研究突出了苹果P. expansum感染期间的差异代谢物,并为进一步调查提供了基础。

更新日期:2021-07-14
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