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Optimization of enzymatic hydrolysis conditions of chemical pretreated cotton stalk using response surface methodology for enhanced bioethanol production yield
Biomass Conversion and Biorefinery ( IF 4 ) Pub Date : 2021-07-14 , DOI: 10.1007/s13399-021-01692-7
Oznur Yildirim 1 , Dogukan Tunay 1 , Bestami Ozkaya 1
Affiliation  

Lignocellulosic biomass is a promising biofuel source that is studied for recent years. However, the biofuel potential of lignocellulosic biomass relies on the pretreatment performance directly and sugar recovery yields, and the pretreatment processes required to achieve that yield are significant. Therefore, several pretreatment methods (acid, alkaline, liquid hot water, and enzymatic hydrolysis) were investigated in different conditions (temperature, enzyme load, and duration) to determine mathematically and statistically best conditions. In the results obtained from this study, acid pretreatment (2% v/v) had the highest effect for obtaining reducing sugar from cellulose, hemicellulose, and lignin compared to liquid hot water (150 °C) and base (0.75% w/v) pretreatments from cotton stalk. According to the response surface methodology analysis, the most efficient achievable enzymatic hydrolysis load was 40 FPU/g biomass with 65-h retention time in 45 °C. The highest total reducing sugar yield was found as 30 g fermentable sugar/L or 0.36 g sugar/g biomass, and the bioethanol concentration was found as 9.5 g/L.

Graphical abstract



中文翻译:

响应面法优化化学预处理棉秆酶解条件提高生物乙醇产量

近年来,木质纤维素生物质是一种很有前途的生物燃料来源。然而,木质纤维素生物质的生物燃料潜力直接依赖于预处理性能和糖回收率,实现该产量所需的预处理过程非常重要。因此,在不同条件(温度、酶负荷和持续时间)下研究了几种预处理方法(酸、碱、液体热水和酶水解),以确定数学和统计上的最佳条件。在本研究中获得的结果中,与液态热水 (150 °C) 和碱 (0.75% w/v) 相比,酸预处理 (2% v/v) 对从纤维素、半纤维素和木质素中获得还原糖的效果最好) 来自棉秆的预处理。根据响应面法分析,最有效的酶水解负荷是 40 FPU/g 生物质,在 45 °C 下保留时间为 65 小时。发现最高总还原糖产量为 30 g 可发酵糖/L 或 0.36 g 糖/g 生物质,生物乙醇浓度为 9.5 g/L。

图形概要

更新日期:2021-07-14
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