The purpose of the study was to clone the sequences of myogenic regulatory factors in Acipenser dabryanus and explore the changes in their expression during starvation and refeeding in A. dabryanus muscle. One hundred twenty fish (60.532 ± 0.284 g) were randomly assigned to four groups (fasted for 0, 3, 7 or 14 d and then refed for 14 d). Our predictions showed that the coding sequences of myod1, myf5, myog and myf6 in A. dabryanus encoded 275, 248, 248 and 243 amino acids, respectively, and the expression of the four genes was the highest in muscle. During fasting, the expression of myod1 in muscle was significantly decreased in the 14 d group. The expressions of myf5 and myf6 were increased significantly at first and then decreased with prolonged starvation time. The expression of myog in the 14 d group was significantly decreased compared with other groups (P < 0.05). During refeeding, the highest values of myod1 and myf6 expression were found in the 3 d group (P < 0.05).The expressions of myf5 and myog in 0 d and 3 d group were significantly higher than those in 7 d and 14 d group (P < 0.05). These results indicate that myogenic regulatory factors (MRFs) play important roles in muscle growth and development in A. dabryanus. The inhibition of long-term starvation (14 d) on the expression of myogenic regulatory factors is probably one of the reasons why it can not achieve full compensation for growth.

本研究的目的是克隆Acipenser dabryanus中生肌调节因子的序列，并探讨其在Acipenser dabryanus肌肉饥饿和重新进食期间表达的变化。一百二十条鱼 (60.532 ± 0.284 g) 被随机分配到四组（禁食 0、3、7 或 14 天，然后再进食 14 天）。我们的预测表明的编码序列MYOD1，Myf5的，MyoG基因和myf6在A.鲟分别编码275，248，248和243个氨基酸，以及四个基因的表达是在肌肉中最高的。禁食期间myod1的表达14 d 组肌肉显着下降。myf5和myf6的表达随饥饿时间的延长呈先显着升高后降低的趋势。与其他组相比，第14 d组myog表达明显降低（P  < 0.05）。再喂养期间，myod1和myf6的表达值在3 d组最高（P  < 0.05）。myf5和myog在0 d和3 d组的表达显着高于7 d和14 d组（磷 < 0.05)。这些结果表明，肌源性调节因子 (MRF) 在A. dabryanus 的肌肉生长和发育中起重要作用。长期饥饿（14 d）对生肌调节因子表达的抑制可能是其无法实现生长完全补偿的原因之一。