当前位置: X-MOL 学术Biomol. Biomed. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Long noncoding RNA FAM225B facilitates proliferation and metastasis of nasopharyngeal carcinoma cells by regulating miR-613/CCND2 axis.
Biomolecules and Biomedicine ( IF 3.4 ) Pub Date : 2021-06-17 , DOI: 10.17305/bjbms.2021.5691
Weijun Dai 1 , Yi Shi 2 , Weiqi Hu 2 , Chenjie Xu 3
Affiliation  

Growing evidence has suggested that abnormally expressed long non-coding RNAs (lncRNAs) play critical regulatory roles in nasopharyngeal carcinoma (NPC) pathogenesis. Family with sequence similarity 225 member B (FAM225B) is a novel lncRNA that has been implicated in several human cancers, yet its role in the context of NPC remains largely unclear. The aim of this study was to determine the expression level of FAM225B and its clinical significance in NPC patients. We observed a remarkable increase of FAM225B in NPC tissues and cell lines compared with controls. Also, highly expressed FAM225B was closely correlated with advanced TNM stage, distant metastasis, and poor overall survival. Interestingly, loss-of-function analysis revealed that FAM225B knockdown significantly inhibited tumor growth in vitro and in vivo, and decreased the migratory and invasive capacity of NPC cells. Mechanically, FAM225B functioned as an endogenous sponge by competing for miR-613 binding to up-regulate CCND2 expression. More importantly, rescue experiments further demonstrated that the suppressive impacts of FAM225B knockdown on cell proliferation, migration and invasion were significantly reversed after CCND2 overexpression. Taken all together, these findings highlight FAM225B as an oncogene that promotes NPC proliferation and metastasis through miR-613/CCND2 axis.

中文翻译:

长链非编码 RNA FAM225B 通过调节 miR-613/CCND2 轴促进鼻咽癌细胞的增殖和转移。

越来越多的证据表明,异常表达的长链非编码 RNA (lncRNA) 在鼻咽癌 (NPC) 发病机制中起关键的调节作用。具有序列相似性 225 成员 B (FAM225B) 的家族是一种新型 lncRNA,它与几种人类癌症有关,但其在 NPC 中的作用仍不清楚。本研究的目的是确定 FAM225B 的表达水平及其在 NPC 患者中的临床意义。与对照相比,我们观察到 NPC 组织和细胞系中 FAM225B 的显着增加。此外,高表达的 FAM225B 与晚期 TNM 分期、远处转移和较差的总生存期密切相关。有趣的是,功能丧失分析显示 FAM225B 敲低在体外和体内显着抑制肿瘤生长,并降低NPC细胞的迁移和侵袭能力。机械地,FAM225B 通过竞争 miR-613 结合上调 CCND2 表达而起到内源海绵的作用。更重要的是,救援实验进一步证明,在 CCND2 过表达后,FAM225B 敲低对细胞增殖、迁移和侵袭的抑制作用显着逆转。总之,这些发现强调了 FAM225B 作为一种致癌基因,通过 miR-613/CCND2 轴促进 NPC 增殖和转移。CCND2过表达后迁移和侵袭显着逆转。总之,这些发现强调了 FAM225B 作为一种致癌基因,通过 miR-613/CCND2 轴促进 NPC 增殖和转移。CCND2过表达后迁移和侵袭显着逆转。总之,这些发现强调了 FAM225B 作为一种致癌基因,通过 miR-613/CCND2 轴促进 NPC 增殖和转移。
更新日期:2021-07-15
down
wechat
bug