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Eco-friendly degradative decolourisation and detoxification of azo dye Amido Black 10B by Bacillus sp. strain AK1
Chemistry and Ecology ( IF 2.5 ) Pub Date : 2021-07-13 , DOI: 10.1080/02757540.2021.1951257
Ramesh S. Masarbo 1 , Joan Chebet 2 , H. C. Shrishail 3 , T. B. Karegoudar 4
Affiliation  

ABSTRACT

In this study, an azo dye Amido Black 10B was decolourised and degraded by using bacterium Bacillus sp. strain AK1. The strain decolourised 96% of the dye in 30 h at 100 mg L−1 dye concentration. Decolourisation under different sets of conditions, such as increasing dye concentration (100–600 mg L−1), pH (5–11), temperature (25–55°C) and salt levels (10–40 g L−1), was studied and found that the strain could decolourise up to 600 mg L−1 of the dye by about 54% in 72 h at pH 7 and 35°C. The bacterium also exhibited the ability to act under varied pH from 5 to 11, temperature from 25°C to 55°C and salt levels of up to 30 g L−1. The azoreductase enzyme responsible for the breaking of azo bond exhibited the activity of 1.5371 µM min−1 mg−1 protein. The products of dye degradation were identified by using UV–Visible spectroscopy and HPLC. The decolourisation also resulted in a decline in toxicity, as indicated by phytotoxicity assessment. There was also a corresponding decrease in the BOD and COD of the decolourised sample by more than 85%, which indicates the reduction in the pollution loads after decolourisation.



中文翻译:

芽孢杆菌对偶氮染料 Amido Black 10B 的环保降解脱色和解毒。菌株AK1

摘要

在这项研究中,偶氮染料 Amido Black 10B 通过使用细菌芽孢杆菌进行脱色和降解。菌株 AK1。在 100 mg L -1染料浓度下,该菌株在 30 小时内使 96% 的染料脱色。在不同条件下脱色,例如增加染料浓度(100-600 mg L -1)、pH(5-11)、温度(25-55°C)和盐含量(10-40 g L -1),研究发现,在 pH 7 和 35°C 下,该菌株可在 72 小时内使多达 600 mg L -1的染料脱色约 54%。该细菌还表现出在 5 到 11 的不同 pH、25°C 到 55°C 的温度和高达 30 g L -1 的盐含量下起作用的能力. 负责破坏偶氮键的偶氮还原酶表现出1.5371 μM min -1  mg -1蛋白质的活性。染料降解产物通过紫外-可见光谱和高效液相色谱法进行鉴定。植物毒性评估表明,脱色还导致毒性下降。脱色样品的 BOD 和 COD 也相应下降了 85% 以上,表明脱色后污染负荷减少。

更新日期:2021-08-01
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