ABSTRACT

Oxidative stress is known to be involved in development of numerous diseases including cardiovascular, respiratory, renal, kidney and cancer. Thus, investigations that mimic oxidative stress in vitro may play an important role to find new strategies to control oxidative stress and subsequent consequences are important. Rotenone, widely used as a pesticide has been used as a model to simulate oxidative stress. However, this chemical was found to produce several diseases. Therefore, the aim of this study was to investigate the antioxidant and cytoprotective effect of avocado (Persea americana Mill) extract and oil in monkey kidney epithelial cells (VERO) exposed to rotenone. VERO cells were exposed to IC₅₀ of rotenone in conjunction with different concentrations of avocado extract and oil (ranging from 1 to 1000 µg/ml), for 24 hr. Subsequently, cell viability and oxidative metabolism were assessed. Data demonstrated that avocado extract and oil in the presence of rotenone increased cellular viability at all tested concentrations compared to cells exposed only to rotenone. In addition, extract and avocado oil exhibited antioxidant action as evidenced by decreased levels of reactive oxygen species (ROS), superoxide ion, and lipid peroxidation, generated by rotenone. Further, avocado extract and oil appeared to be safe, since these compounds did not affect cell viability and or generate oxidative stress. Therefore, avocado appears to display a promising antioxidant potential by decreasing oxidative stress.

摘要

已知氧化应激与多种疾病的发展有关，包括心血管、呼吸系统、肾脏、肾脏和癌症。因此，在体外模拟氧化应激的研究可能在寻找控制氧化应激和后续后果的新策略方面发挥重要作用。鱼藤酮广泛用作杀虫剂，已被用作模拟氧化应激的模型。然而，发现这种化学物质会产生多种疾病。因此，本研究的目的是研究鳄梨 ( Persea americana Mill) 提取物和油对暴露于鱼藤酮的猴肾上皮细胞 (VERO)的抗氧化和细胞保护作用。VERO 细胞暴露于 IC ₅₀鱼藤酮与不同浓度的鳄梨提取物和油（范围从 1 到 1000 µg/ml）相结合，持续 24 小时。随后，评估细胞活力和氧化代谢。数据表明，与仅暴露于鱼藤酮的细胞相比，存在鱼藤酮的鳄梨提取物和油在所有测试浓度下均增加了细胞活力。此外，鱼藤酮产生的活性氧 (ROS)、超氧离子和脂质过氧化水平降低，证明提取物和鳄梨油具有抗氧化作用。此外，鳄梨提取物和油似乎是安全的，因为这些化合物不会影响细胞活力和/或产生氧化应激。因此，鳄梨似乎通过降低氧化应激显示出有希望的抗氧化潜力。