Abstract

Apocynum venetum is a kind of plant with high medicinal value. Its main component is rutin, which is widely used. In the present study, an efficient method based on high-speed counter-current chromatography (HSCCC) was established to separate rutin from A. venetum. The optimized solvent system composed of ethyl acetate-n-butanol-water (4:1:5, v/v) was selected for HSCCC separation. It was found that the maximum loading amount was 1 g, but the loading amount did not affect the two-phase equilibrium of solvent system. Therefore, consecutive high-speed counter-current chromatography was introduced to further improve the yield in the present study. As a result, 292 mg of rutin with 95% purity was obtained from 2.4 g crude sample. The retention of the stationary phase was 27%, which was almost unchanged. The current strategy can not only be used for the scale-up preparation of rutin, but also provide an example for the preparation of other natural active compounds.

摘要

罗布麻是一种药用价值很高的植物。其主要成分是芦丁，用途广泛。在本研究中，建立了一种基于高速逆流色谱 (HSCCC) 的有效方法来从A. venetum 中分离芦丁。优化后的乙酸乙酯-正丁醇-水(4:1:5, v/v) 被选择用于 HSCCC 分离。发现最大加载量为1 g，但加载量不影响溶剂体系的两相平衡。因此，在本研究中引入连续高速逆流色谱以进一步提高产率。结果，从 2.4 g 粗样品中获得了 292 mg 纯度为 95% 的芦丁。固定相的保留率为 27%，几乎没有变化。目前的策略不仅可以用于芦丁的放大制备，还可以为其他天然活性化合物的制备提供范例。