Abstract

Several species of Cryptocarya (Lauraceae) are traditionally used in folk medicines for the treatment of various diseases and disorders in tropical and subtropical countries. The essential oil extracted from the leaves and barks of Cryptocarya species are reported to possess antimicrobial, insecticidal, antioxidant, and antitumoral activities. However, there are no scientific reports on the biological activity of essential oil extracted from the tropical Asiatic species Cryptocarya amygdalina. The present study was undertaken to determine the chemical composition and evaluate the antioxidant, anticancer, and anti-inflammatory activities of bark essential oil of C. amygdalina. A total of 57 constituents accounting for 99.05 % of the total essential oil content were identified by GC/MS. The bark essential oil was dominated by sesquiterpene hydrocarbons (78.71 %) with β- caryophyllene (34.77 %), α-copaene (14.26 %), bicyclogermacrene (7.65 %), and δ-cadinene (4.42 %) as the major constituents. The essential oil exhibited good radical scavenging activity against DPPH, ABTS, and Reducing power assay with half-maximal inhibitory concentration (IC₅₀) values of 6.97 ± 0.24, 1.28 ± 0.07, and 9.23 ± 0.34 μg/mL, respectively compared to ascorbic acid and BHT. The Pearson’s coefficient analysis showed a significant correlation between TPC, TFC, and an antioxidant assay of C. amygdalina essential oil. The essential oil also showed potent cytotoxicity against human colon (HCT-116) and ovarian (PA-1) cancer cell lines with IC₅₀ values lesser than 50 μg/mL in both cases. Besides, this bark essential oil at 50 μg/mL concentration exhibited significant anti-inflammatory activity in lipopolysaccharide-activated RAW 264.7 macrophages by inhibiting nitric oxide production.

摘要

在热带和亚热带国家，传统上将几种Cryptocarya（月桂科）用于治疗各种疾病和紊乱的民间药物。据报道，从Cryptocarya物种的叶子和树皮中提取的精油具有抗菌、杀虫、抗氧化和抗肿瘤活性。然而，从热带亚洲物种Cryptocarya amygdalina 中提取的精油的生物活性尚无科学报告。本研究旨在确定苦杏仁树皮精油的化学成分并评估其抗氧化、抗癌和抗炎活性. GC/MS共鉴定出57种成分，占精油总含量的99.05%。树皮精油以倍半萜烃（78.71%）为主，主要成分为β-石竹烯（34.77%）、α-可帕烯（14.26%）、双环锗烯（7.65%）和δ-卡二烯（4.42%）。与抗坏血酸相比，精油对 DPPH、ABTS 和还原能力试验表现出良好的自由基清除活性，半数抑制浓度 (IC ₅₀ ) 值分别为 6.97 ± 0.24、1.28 ± 0.07 和 9.23 ± 0.34 μg/mL和 BHT。Pearson 系数分析显示 TPC、TFC 和C. amygdalina的抗氧化检测之间存在显着相关性香精油。在两种情况下，精油还对人结肠 (HCT-116) 和卵巢 (PA-1) 癌细胞系显示出有效的细胞毒性，IC ₅₀值均小于 50 μg/mL。此外，这种 50 μg/mL 浓度的树皮精油通过抑制一氧化氮的产生，在脂多糖激活的 RAW 264.7 巨噬细胞中表现出显着的抗炎活性。