Poly(N-isopropylacrylamide) (PNIPAAm) is the most well-known and widely used stimuli-responsive polymer in the biomedical field owing to its ability to undergo temperature-dependent hydration and dehydration with temperature variations, causing hydrophilic and hydrophobic alterations. This temperature-dependent property of PNIPAAm provides functionality to interfaces containing PNIPAAm. Notably, the hydrophilic and hydrophobic alterations caused by the change in the temperature-responsive property of PNIPAAm-modified interfaces induce temperature-modulated interactions with biomolecules, proteins, and cells. This intrinsic property of PNIPAAm can be effectively used in various biomedical applications, particularly in bioseparation and tissue engineering applications, owing to the functionality of PNIPAAm-modified interfaces based on the temperature modulation of the interaction between PNIPAAm-modified interfaces and biomolecules and cells. This review focuses on PNIPAAm-modified interfaces in terms of preparation method, properties, and their applications. Advances in PNIPAAm-modified interfaces for existing and developing applications are also summarized.

聚( N-异丙基丙烯酰胺) (PNIPAAm) 是生物医学领域中最著名和应用最广泛的刺激响应聚合物，因为它能够随温度变化而发生依赖于温度的水合作用和脱水，从而引起亲水和疏水变化。PNIPAAm 的这种温度相关特性为包含 PNIPAAm 的接口提供了功能。值得注意的是，由 PNIPAAm 修饰界面的温度响应特性的变化引起的亲水和疏水改变会诱导与生物分子、蛋白质和细胞的温度调节相互作用。PNIPAAm 的这种固有特性可以有效地用于各种生物医学应用，特别是在生物分离和组织工程应用中，由于 PNIPAAm 修饰界面的功能基于 PNIPAAm 修饰界面与生物分子和细胞之间相互作用的温度调制。本综述重点介绍了 PNIPAAm 改性界面的制备方法、性能及其应用。还总结了用于现有和开发应用程序的 PNIPAAm 修改接口的进展。