Decreased expression of miR-142-3p was observed in human cancers. However, the function and mechanism of miR-142-3p in human colorectal cancer remain obscure. The expressions of miR-142-3p in human colorectal cancer tissues and cell lines were measured by RT-qPCR. The effects of miR-142-3p on cell invasion and migration were detected by transwell assays. The efficiency of aerobic glycolysis was determined by glucose consumption and lactate production. Dual-luciferase reporter assays were performed to confirm the correlation between miR-142-3p and pyruvate kinase isozyme M2 (PKM2). The level of PKM2 was assessed by western blotting. Our results showed that the expression of miR-142-3p was decreased both in human colorectal cancer tissues and in cells. Overexpression of miR-142-3p in cell line attenuated colorectal cancer cell invasion and migration. About the underlying mechanism, we found that miR-142-3p modulated aerobic glycolysis via targeting pyruvate kinase M2 (PKM2). In addition, we demonstrated PKM2 and PKM2-mediated aerobic glycolysis contributes to miR-142-3p-mediated colorectal cancer cell invasion and migration. Hence, these data suggested that miR-142-3p was a potential therapeutic target for the treatment of human colorectal cancer.

中文翻译：

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miR-142-3p 通过 PKM2 介导的有氧糖酵解调节结直肠癌中的细胞侵袭和迁移

在人类癌症中观察到 miR-142-3p 的表达降低。然而，miR-142-3p在人结直肠癌中的作用和机制仍不清楚。RT-qPCR检测miR-142-3p在人结直肠癌组织和细胞系中的表达。通过transwell测定检测miR-142-3p对细胞侵袭和迁移的影响。有氧糖酵解的效率取决于葡萄糖消耗和乳酸产生。进行双荧光素酶报告基因测定以确认 miR-142-3p 和丙酮酸激酶同工酶 M2 (PKM2) 之间的相关性。通过蛋白质印迹评估PKM2的水平。我们的结果表明，miR-142-3p 在人结直肠癌组织和细胞中的表达均降低。miR-142-3p 在细胞系中的过表达减弱了结直肠癌细胞的侵袭和迁移。关于潜在机制，我们发现 miR-142-3p 通过靶向丙酮酸激酶 M2 (PKM2) 调节有氧糖酵解。此外，我们证明了 PKM2 和 PKM2 介导的有氧糖酵解有助于 miR-142-3p 介导的结直肠癌细胞侵袭和迁移。因此，这些数据表明 miR-142-3p 是治疗人类结直肠癌的潜在治疗靶点。