当前位置: X-MOL 学术Respir. Physiol. Neurobiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Analysis of miRNA expression profile in lung tissues of an intermittent hypoxia rat model
Respiratory Physiology & Neurobiology ( IF 2.3 ) Pub Date : 2021-07-14 , DOI: 10.1016/j.resp.2021.103741
Zhijuan Liu 1 , Li Ai 2 , Ran Li 2 , Yuan Yang 2 , Keli Chen 2 , Chunxia He 1 , Yongxia Li 2
Affiliation  

We screened key miRNAs in an intermittent hypoxia rat model and explored the biological roles of downstream target genes and related regulatory pathways. We analyzed the expression profile of miRNAs in the lung tissues of rats in the 5 % (IH1), 7.5 % (IH2), 10 % (IH3), 12.5 % (IH4) oxygen concentration and negative control (NC) groups and identified common miRNAs. Multiple differentially expressed miRNAs were detected, and intersection of their expression profiles yielded 10 common miRNAs with 929 target genes mainly distributed in the nucleus. Molecular functions pertained mainly to the activation of transcription factors, while biological processes focused on cell interaction and signal transduction. Among signaling pathways, the top 5 included the LKB1 signaling, nectin adhesion, and S1P pathways. 8 of 10 common miRNAs had excellent diagnostic value for detecting intermittent hypoxia. The miRNAs binds to the target gene might play a key role in the pathophysiological process of OSA through the LKB1/AMPK and S1P/Akt/eNOS signaling pathways.



中文翻译:

间歇性缺氧大鼠模型肺组织中miRNA表达谱分析

我们筛选了间歇性缺氧大鼠模型中的关键 miRNA,并探索了下游靶基因和相关调控途径的生物学作用。我们分析了 5% (IH1)、7.5% (IH2)、10% (IH3)、12.5% (IH4) 氧浓度和阴性对照组 (NC) 组大鼠肺组织中 miRNA 的表达谱,并确定了常见的miRNA。检测到多个差异表达的miRNA,将它们的表达谱交叉产生10个常见的miRNA,其中929个靶基因主要分布在细胞核中。分子功能主要与转录因子的激活有关,而生物过程则集中在细胞相互作用和信号转导上。在信号通路中,前 5 位包括 LKB1 信号通路、nectin 粘附通路和 S1P 通路。10 种常见 miRNA 中有 8 种对检测间歇性缺氧具有极好的诊断价值。miRNAs与靶基因结合可能通过LKB1/AMPK和S1P/Akt/eNOS信号通路在OSA的病理生理过程中发挥关键作用。

更新日期:2021-07-21
down
wechat
bug