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Characterization and genome analysis of novel Klebsiella phage BUCT556A with lytic activity against carbapenemase-producing Klebsiella pneumoniae
Virus Research ( IF 5 ) Pub Date : 2021-07-13 , DOI: 10.1016/j.virusres.2021.198506
Jiao Feng 1 , Liting Gao 1 , Lu Li 2 , Zhijun Zhang 3 , Changxin Wu 1 , Fei Li 4 , Yigang Tong 5
Affiliation  

Carbapenem-resistant Klebsiella pneumoniae (CRKP) have spread globally and led to the limited choice of antimicrobial treatment of K. pneumoniae-induced infections. Bacteriophages are considered as an effective strategy against bacterial infections. In this study, we isolated a novel Klebsiella phage BUCT556A with lytic activity against KPC-producing K. pneumoniae, which was a multi-drug resistant isolate. Phage BUCT556A had a symmetrical head and a long, non-contractile tail, belonging to the family Siphoviridae, order Caudoviridae. Phage BUCT556A had a relatively narrow host range, and a medium burst size of 91 PFU/cell. It was stable at broad temperature/pH range, and exhibited good tolerance to chloroform. The genome of phage BUCT556A was a 49, 376-bp linear double-stranded DNA molecule with average G + C content of 50.2%, and contained 75 open reading frames. There was no tRNA, antibiotic resistance, toxin, virulence related genes or lysogen-formation gene clusters detected in the genome of phage BUCT556A. Phylogenetic analyses based on the major capsid protein Mcp suggested that this phage had a close relationship with Klebsiella phage KLPN1. Together, through phenotypic combined with genomic DNA sequencing and analyses, our study suggests that phage BUCT556A has the potential to be used as a bacterial treatment tool for multidrug-resistant strains K. pneumoniae.



中文翻译:

对产碳青霉烯酶肺炎克雷伯菌具有裂解活性的新型克雷伯菌噬菌体 BUCT556A 的表征和基因组分析

耐碳青霉烯类肺炎克雷伯菌(CRKP) 已在全球蔓延,导致对肺炎克雷伯菌引起的感染的抗菌治疗选择有限。噬菌体被认为是对抗细菌感染的有效策略。在这项研究中,我们分离出一种新型克雷伯氏菌噬菌体 BUCT556A,该噬菌体对产生 KPC 的肺炎克雷伯菌具有裂解活性,它是一种多重耐药分离株。噬菌体 BUCT556A 有一个对称的头部和一个长的、非收缩的尾巴,属于Siphoviridae, order Caudoviridae. 噬菌体 BUCT556A 的宿主范围相对较窄,中等爆发大小为 91 PFU/细胞。它在较宽的温度/pH 范围内稳定,对氯仿表现出良好的耐受性。噬菌体BUCT556A基因组为49、376-bp线性双链DNA分子,平均G+C含量为50.2%,包含75个开放阅读框。噬菌体BUCT556A基因组中未检测到tRNA、抗生素抗性、毒素、毒力相关基因或溶原形成基因簇。基于主要衣壳蛋白Mcp的系统发育分析表明,该噬菌体与克雷伯氏菌有密切的关系。噬菌体KLPN1。总之,通过表型结合基因组 DNA 测序和分析,我们的研究表明噬菌体 BUCT556A 有可能用作多药耐药菌株肺炎克雷伯菌的细菌治疗工具。

更新日期:2021-07-20
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