The genus Flavivirus incorporates bona fide arboviruses, as well as others viruses with restricted replication in insect cells. Among the latter, a large monophyletic cluster of viruses, known as cISF (classical insect-specific flaviviruses), has been sampled in many species of mosquitoes collected over a large geographic range.

In this study, we investigated nucleotide and protein sequences with a suite of molecular characterization approaches including genetic distance, Shannon entropy, selective pressure analysis, polymorphism identification, principal coordinate analysis, likelihood mapping, phylodynamic reconstruction, and spatiotemporal dispersal, to further characterize this diverse group of insect-viruses. The different lineages and sub-lineages of viral sequences presented low sequence diversity and entropy (though some displayed lineage-specific polymorphisms), did not show evidence of frequent recombination and evolved under strong purifying selection. Moreover, the reconstruction of the evolutionary history and spatiotemporal dispersal was highly impacted by overall low signals of sequence divergence throughout time but suggested that cISF distribution in space and time is dynamic and may be dependent on human activities, including commercial trading and traveling.

黄病毒属包含真正的虫媒病毒，以及其他在昆虫细胞中复制受限的病毒。在后者中，一个大的单系病毒群，称为 cISF（经典昆虫特异性黄病毒），已在广泛地理范围内收集的许多蚊子种类中取样。

在这项研究中，我们使用一套分子表征方法研究了核苷酸和蛋白质序列，包括遗传距离、香农熵、选择性压力分析、多态性识别、主坐标分析、似然图、系统动力学重建和时空扩散，以进一步表征这种多样化的一组昆虫病毒。病毒序列的不同谱系和亚谱系呈现出低序列多样性和熵（尽管一些显示谱系特异性多态性），没有显示频繁重组的证据并且在强纯化选择下进化。而且，