Sensitive methods, capable of rapidly and accurately detecting aquatic invasive species, are in demand. Molecular-based approaches, such as environmental DNA (eDNA) surveys, satisfy these requirements and have grown in popularity. As such, eDNA surveys could aid the effort to combat the colonisation and spread of two notoriously invasive freshwater mussel species, the quagga mussel (Dreissena rostriformis bugensis) and zebra mussel (D. polymorpha), through improved surveillance ability. Here, we provide a review of dreissenid eDNA literature (both grey and published), summarising efforts involved in the development of various assays for use in multiple different technologies (e.g. quantitative PCR, high-throughput sequencing and loop-mediated isothermal amplification) and sampling scenarios. We discuss important discoveries made along the way, including novel revelations involving environmental RNA (eRNA), as well as the advantages and limitations of available methods and instrumentation. In closing, we highlight critical remaining gaps, where further investigation could lead to advancements in dreissenid monitoring capacity.

中文翻译：

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斑驴和斑马贻贝的先进分子监测：环境 DNA/RNA (eDNA/eRNA) 研究综述和未来方向的考虑

需要能够快速准确地检测水生入侵物种的敏感方法。基于分子的方法，例如环境 DNA (eDNA) 调查，满足这些要求并且越来越受欢迎。因此，eDNA 调查可以通过提高监测能力，帮助打击两种臭名昭著的入侵淡水贻贝物种——斑贻贝 (Dreissena rostriformis bugensis) 和斑马贻贝 (D. polymorpha) 的定殖和传播。在这里，我们提供了德莱森 eDNA 文献（灰色和已发表）的综述，总结了用于多种不同技术（例如定量 PCR、高通量测序和环介导等温扩增）和采样的各种检测方法的开发工作场景。我们讨论沿途取得的重要发现，包括涉及环境 RNA (eRNA) 的新发现，以及可用方法和仪器的优点和局限性。最后，我们强调了关键的剩余差距，进一步调查可能会导致德莱森监测能力的进步。