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Persistence of undifferentiated spermatogonia in aged Japanese Black cattle
Animal Science Journal ( IF 2 ) Pub Date : 2021-07-12 , DOI: 10.1111/asj.13572
Terumichi Kawahara 1 , Miki Kanouchi 1 , Yousuke Naniwa 2 , Masanori Koyago 2 , Takashi Numabe 3 , Keishi Mizutani 4 , Kentaro Tanemura 1 , Kenshiro Hara 1
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Aging is a major risk factor for spermatogenesis deterioration. However, the influence of age on spermatogenic stem cells and their progenitors in bulls is largely unknown. Here, we report age-related changes in undifferentiated and differentiating spermatogonia in Japanese Black cattle with nearly constant sperm output, by using spermatogonial markers. The numbers of differentiating spermatogonia and more differentiated spermatogenic cells were significantly decreased in aged bovine testes compared with those in young testes. In contrast, the number of undifferentiated spermatogonia was maintained, and their proliferative activity did not differ significantly between young and aged bovine testes. Although severe calcification was only observed to a small extent in aged testes, fewer Sertoli cells and interstitial fibrosis were observed in noncalcified testicular regions. These results suggest that, even in old bulls with nearly constant sperm output, testicular spermatogenic activity declined whereas undifferentiated spermatogonia numbers were maintained. Thus, we propose that undifferentiated spermatogonia may be resistant to age-related changes in bovine testes. Because undifferentiated spermatogonia may contain stem cell activity, our findings highlight the potential utility of undifferentiated spermatogonia as an agricultural resource to produce spermatozoa beyond the natural bovine lifetime through transplantation and in vitro spermatogenesis in future animal production.

中文翻译:

老年日本黑牛未分化精原细胞的持续存在

衰老是精子发生恶化的主要危险因素。然而,年龄对公牛生精干细胞及其祖细胞的影响在很大程度上是未知的。在这里,我们通过使用精原细胞标记物报告了精子产量几乎恒定的日本黑牛未分化和分化精原细胞的年龄相关变化。与年轻牛睾丸相比,老年牛睾丸中分化的精原细胞和更多分化的生精细胞的数量显着减少。相反,未分化精原细胞的数量保持不变,并且它们的增殖活性在年轻和年老的牛睾丸之间没有显着差异。尽管在老年睾丸中仅在很小程度上观察到严重的钙化,在未钙化的睾丸区域观察到较少的支持细胞和间质纤维化。这些结果表明,即使在精子输出量几乎恒定的老年公牛中,睾丸生精活性也会下降,而未分化的精原细胞数量会保持不变。因此,我们提出未分化的精原细胞可能对牛睾丸中与年龄相关的变化有抵抗力。因为未分化的精原细胞可能含有干细胞活性,我们的研究结果强调了未分化精原细胞作为农业资源的潜在用途,可以在未来的动物生产中通过移植和体外精子发生来生产超过自然牛寿命的精子。睾丸生精活性下降,而未分化的精原细胞数量保持不变。因此,我们提出未分化的精原细胞可能对牛睾丸中与年龄相关的变化有抵抗力。因为未分化的精原细胞可能含有干细胞活性,我们的研究结果强调了未分化精原细胞作为农业资源的潜在用途,可以在未来的动物生产中通过移植和体外精子发生来生产超过自然牛寿命的精子。睾丸生精活性下降,而未分化的精原细胞数量保持不变。因此,我们提出未分化的精原细胞可能对牛睾丸中与年龄相关的变化有抵抗力。因为未分化的精原细胞可能含有干细胞活性,我们的研究结果强调了未分化精原细胞作为农业资源的潜在用途,可以在未来的动物生产中通过移植和体外精子发生来生产超过自然牛寿命的精子。
更新日期:2021-07-13
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