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Secretome characterization of the lignocellulose-degrading fungi Pycnoporus sanguineus and Ganoderma resinaceum growing on Panicum prionitis biomass
Mycologia ( IF 2.8 ) Pub Date : 2021-07-12 , DOI: 10.1080/00275514.2021.1922249
Albertina Gauna 1 , Alvaro S Larran 1, 2 , Susana R Feldman 1, 2, 3 , Hugo R Permingeat 1, 2 , Valeria E Perotti 1
Affiliation  

ABSTRACT

C4 grasses are common species in rangelands around the world and represent an attractive option for second-generation biofuel production. Although they display high polysaccharide content and reach great levels of biomass accumulation, there is a major technical issue to be addressed before they can be used for bioethanol industrial production: lignin removal. Concerning this, Pycnoporus and Ganoderma fungal genera have been highlighted due to their ability to hydrolyze lignocellulose in biological pretreatments. Our goals here were to evaluate the pretreatment efficiency using the secretome of species from Pycnoporus and Ganoderma spp. harvested from a glucose-free inductive medium (using a C4 grass) and to identify the fungal enzymatic activities responsible for the lignin degradation and glucose release. Our results show that P. sanguineus secretome exhibits a higher activity of lignocellulolytic enzymes such as cellulases, xylanases, laccases, and manganese peroxidases compared with that from G. resinaceum. Interestingly, zymograms in the presence of 2 M glucose suggest that a β-glucosidase isoform from P. sanguineus could be glucose tolerant. The proteomic approach carried out allowed the identification of 73 and 180 different proteins in G. resinaceum and P. sanguineus secretomes, respectively, which were functionally classified in five main categories and a miscellaneous group. These results open new avenues for future experimental work that lead to a deeper comprehension and a greater application of the mechanisms underlying lignocellulosic biomass degradation.



中文翻译:

生长在Panicum prionitis生物质上的木质纤维素降解真菌Pycnoporus sanguineus和Ganoderma resinaceum的分泌特征

摘要

C4 草是世界各地牧场的常见物种,是第二代生物燃料生产的有吸引力的选择。尽管它们显示出高多糖含量并达到很高的生物质积累水平,但在将它们用于生物乙醇工业生产之前,有一个主要的技术问题需要解决:木质素去除。关于这一点,PycnoporusGanoderma真菌属因其在生物预处理中水解木质纤维素的能力而受到重视。我们的目标是使用PycnoporusGanoderma物种的分泌组评估预处理效率属 从无葡萄糖诱导培养基(使用 C4 草)中收获,并确定负责木质素降解和葡萄糖释放的真菌酶活性。我们的结果表明,与来自G.resinaceum 的相比,P. sanguineus分泌组表现出更高的木质纤维素分解酶活性,如纤维素酶、木聚糖酶、漆酶和锰过氧化物酶。有趣的是,存在 2 M 葡萄糖时的酶谱表明来自血红假单胞菌的 β-葡萄糖苷酶同种型可能具有葡萄糖耐受性。进行蛋白质组的方法使73个180不同的蛋白质在识别G. resinaceumP.蜱分别在功能上分为五个主要类别和一个杂项组。这些结果为未来的实验工作开辟了新的途径,从而更深入地理解和更好地应用木质纤维素生物质降解的潜在机制。

更新日期:2021-09-16
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