Abstract

Alpinia galanga is commonly known as ‘greater galangal’ is extensively present across India and Southeast Asia. The essential oil of this zingiberaceae species has high demand in the global market as it has a practice in flavoring food throughout Southeast Asia. The essential oil is also used in spa and perfumery. Rhizome essential oil of A. galanga (AGEO) possesses the myriad therapeutic potential and has more yield than leaf essential oil. However, there are no studies available on the anti-microbial activity of nano-emulsified essential oil of A. galanga (AGNO). Thus, in this current study, AGNO was formulated via low energy emulsification method by using tween 20 and isopropanol as surfactant and co-surfactant respectively with water as solvent. Characterization and validation of nano emulsified oil were done by Litesizer 500 which showed its size less than 100 nm. AGNO exhibited enhanced antimicrobial activity (MIC) against different microbial pathogens like Staphylococcus aureus, Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Aspergillus niger, and Fusarium oxysporum when compared with control (AGEO). It has been concluded from antimicrobial activity that nano emulsified essential oil possesses the highest inhibitory activity against Staphylococcus aureus (0.2 μl/ml). The antioxidant ability of the AGEO and AGNO was also compared by using DPPH free radical scavenging assay. The nano emulsified essential oil possessed significantly higher antioxidant activity (IC₅₀-9 μg/ml) as compared to the normal essential oil (IC₅₀-19 μg/ml). Thus, we conclude that the essential oil-based nanoemulsion of A. galanga can be used as an antioxidant and antimicrobial agent in pharmaceutical industries.

摘要

高良姜通常被称为“大高良姜”，广泛存在于印度和东南亚。这种姜科植物的精油在全球市场需求量很大，因为它在整个东南亚地区都有为食物调味的做法。精油也用于水疗和香水。高良姜根茎精油(AGEO) 具有无数的治疗潜力，并且比叶精油产量更高。然而，尚无关于高良姜纳米乳化精油抗微生物活性的研究（阿格诺）。因此，在目前的研究中，AGNO 是通过低能乳化法通过使用吐温 20 和异丙醇分别作为表面活性剂和助表面活性剂，以水为溶剂来配制的。纳米乳化油的表征和验证由 Litesizer 500 完成，显示其尺寸小于 100 nm。与对照 (AGEO) 相比，AGNO对金黄色葡萄球菌、枯草芽孢杆菌、粪肠球菌、大肠杆菌、黑曲霉和尖孢镰刀菌等不同微生物病原体表现出增强的抗菌活性 (MIC )。从抗菌活性得出结论，纳米乳化精油对金黄色葡萄球菌的抑制活性最高(0.2 微升/毫升)。AGEO和AGNO的抗氧化能力也通过DPPH自由基清除试验进行了比较。与普通精油（IC ₅₀ -19 μg/ml）相比，纳米乳化精油具有显着更高的抗氧化活性（IC ₅₀ -9 μg/ml）。因此，我们得出结论，高良姜精油纳米乳剂可用作制药行业的抗氧化剂和抗菌剂。