During the early development of the Pacific cod, high mortality due to viral infection is observed. To demonstrate the immune responses activated in Pacific cod during this critical period, two cytokines, the proinflammatory cytokine interleukin-8 (IL-8) and the anti-inflammatory cytokine interleukin-10 (IL-10), were identified and characterized in this study. The cDNA of IL-8 was 1047 bp in length, containing an ORF of 297 bp that encoded a 98 aa protein, whereas the IL-10 cDNA sequence consisted of 856 bp that harbored a 537 bp ORF encoding a 178 aa protein. Pairwise comparison and phylogenetic analysis of the two genes clearly demonstrated their close relationships with their counterparts in the Atlantic cod. Constitutive expression was detected in eight tested tissues; the transcription of both genes was observed during the critical period of the early developmental stage in Pacific cod, and their expression levels dramatically increased following poly I:C stimulation of larvae at 12 days post-hatching (dph). Taken together, these results suggested that IL-8 and IL-10 may be involved in the inflammatory responses of Pacific cod.

在太平洋鳕鱼的早期发育过程中，观察到病毒感染导致的高死亡率。为了证明太平洋鳕鱼在这一关键时期激活的免疫反应，本研究鉴定并表征了两种细胞因子，促炎细胞因子白介素 8 (IL-8) 和抗炎细胞因子白介素 10 (IL-10) . IL-8 的 cDNA 长度为 1047 bp，包含 297 bp 的 ORF，编码 98 aa 蛋白，而 IL-10 cDNA 序列由 856 bp 组成，包含 537 bp 的 ORF，编码 178 aa 蛋白。这两个基因的成对比较和系统发育分析清楚地证明了它们与大西洋鳕鱼中的对应物的密切关系。在八个测试组织中检测到组成型表达；在太平洋鳕鱼早期发育阶段的关键时期观察到这两种基因的转录，并且在孵化后 12 天 (dph) 对幼虫进行 poly I:C 刺激后，它们的表达水平显着增加。总之，这些结果表明 IL-8 和 IL-10 可能参与了太平洋鳕鱼的炎症反应。