ABSTRACT

We screened 80 Asian rice (Oryza sativa L.) cultivars for the presence of the submergence-tolerance gene SUB1A-1 and the floating genes SNORKEL1 (SK1) and SNORKEL2 (SK2), and found that the deepwater rice cultivar Baisbish (BSB) and the submergence-tolerant cultivar Flood Resistant 13A (FR13A) both possess the SUB1A-1 and the SK1/2. When BSB and FR13A seedlings were completely submerged, spindly growth of shoots was induced in BSB but not in FR13A. Submergence significantly increased the SUB1A-1 transcript abundance in BSB and FR13A shoots, but the expression level in BSB was much lower than that of FR13A. Submergence also induced the expression of both ERF66 and ERF67, the transcriptional targets of SUB1A-1, in FR13A shoots, whereas it upregulated the expression of ERF67 but not that of ERF66 in BSB shoots. These results suggest that BSB could not display submergence tolerance due to the low expression of SUB1A-1 and/or ERF66 under submergence.

摘要

我们对 80 个亚洲水稻 ( Oryza sativa L.) 品种进行了耐淹基因SUB1A-1和漂浮基因SNORKEL1 ( SK1 ) 和SNORKEL2 ( SK2 ) 的筛选，发现深水水稻品种 Baisbish (BSB) 和耐淹品种Flood Resistant 13A（FR13A）均具有SUB1A-1和SK1 / 2。当 BSB 和 FR13A 幼苗完全被淹没时，BSB 中诱导了细长的枝条生长，而 FR13A 中则没有。淹没显着增加了SUB1A-1BSB 和 FR13A 芽中的转录本丰度，但 BSB 中的表达水平远低于 FR13A。淹没还诱导了ERF66和ERF67（SUB1A-1的转录靶标）在FR13A芽中的表达，而它在BSB芽中上调了ERF67的表达但不上调ERF66的表达。这些结果表明，由于SUB1A-1和/或ERF66在淹没下的低表达，BSB 不能显示淹没耐受性。