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Performance of a loop-mediated isothermal amplification assay (Isoplex CRE-ART) to detect common carbapenemase-encoding genes in Gram-negative bacteria
Journal of Medical Microbiology ( IF 3 ) Pub Date : 2021-07-12 , DOI: 10.1099/jmm.0.001379
Laura Berneking 1 , Lucia Asar 1 , Anna Both 1 , Benjamin Berinson 1 , Martin Aepfelbacher 1 , Marc Lütgehetmann 1 , Holger Rohde 1
Affiliation  

Carbapenem-resistant Gram-negative bacteria (CR-GNB) are a major source of nosocomial infections worldwide. In this study, the ability of a loop-mediated isothermal amplification (LAMP)-based method (Isoplex CRE-ART) to rapidly detect carbapenemase-encoding genes bla OXA-48-like, bla OXA-23-like, bla OXA-24-like, bla KPC, bla VIM, bla NDM and bla IMP in 231 carbapenem-resistant Enterobacterales, Pseudomonas aeruginosa and Acinetobacter baumannii isolates was investigated. The accuracy of the LAMP test was compared to results of molecular isolate characterization using a Laboratory Developed Test multiplex carbapenemase PCR assay. The LAMP test correctly identified the presence of on-panel carbapenemases with a sensitivity of 99.16 % [95 % confidence interval (CI): 95.39–99.96 %] and a specificity of 98.21 % (95 % CI: 93.72–99.68 %) in 60 min. Our findings suggest that the Isoplex CRE-ART assay is able to rapidly identify carbapenemase genes in CR-GNB and improves options for pathogen characterization in the context of clinical microbiological and infection control diagnostics.

中文翻译:

环介导等温扩增试验 (Isoplex CRE-ART) 检测革兰氏阴性菌中常见碳青霉烯酶编码基因的性能

耐碳青霉烯革兰氏阴性菌 (CR-GNB) 是全球医院感染的主要来源。在本研究中,基于环介导等温扩增 (LAMP) 的方法 (Isoplex CRE-ART) 能够快速检测碳青霉烯酶编码基因bla OXA-48-likebla OXA-23-likebla OXA-24 -like , bla KPC , bla VIM , bla NDMbla IMP在 231 种耐碳青霉烯类肠杆菌、铜绿假单胞菌鲍曼不动杆菌中 分离株进行了调查。使用实验室开发的测试多重碳青霉烯酶 PCR 测定法将 LAMP 测试的准确性与分子分离物表征的结果进行比较。LAMP 测试以 99.16 % [95 % 置信区间 (CI):95.39–99.96 %] 的敏感性和 98.21 % (95 % CI:93.72–99.68 %) 的特异性在 60 次中正确识别了面板上碳青霉烯酶的存在分钟 我们的研究结果表明,Isoplex CRE-ART 检测能够快速识别 CR-GNB 中的碳青霉烯酶基因,并在临床微生物学和感染控制诊断的背景下改进病原体表征的选择。
更新日期:2021-07-13
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