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Ethylene-induced banana starch degradation mediated by an ethylene signaling component MaEIL2
Postharvest Biology and Technology ( IF 7 ) Pub Date : 2021-07-13 , DOI: 10.1016/j.postharvbio.2021.111648
Li-sha Zhu 1 , Wei Shan 1 , Chao-jie Wu 1 , Wei Wei 1 , Hong Xu 1 , Wang-jin Lu 1 , Jian-ye Chen 1 , Xin-guo Su 2 , Jian-fei Kuang 1
Affiliation  

Starch is the major reserve carbohydrate in nature, which possesses nutritional property and industrial applications. Starch degradation is an important contributor to softening and sweetening of banana fruit, but more details in this process are not fully understood. In this study, the contents of total starch, amylose and amylopectin were gradually decreased during banana fruit ripening, which is in parallel with the increased levels of total soluble sugars. Particularly, reduced sizes and elongated shapes of starch granules, as well as decreased crystallinity were observed as ripening proceeds, which is largely due to the digestion of starch degradation enzymes such as amylase and isoamylase. Importantly, an ethylene signaling component MaEIL2 bound to the promoters of amylase and isoamylase encoding genes MaAMY3, MaISA2 and MaISA3, and stimulated their transcription. Overall, these findings reveal that starch-sugar transformation during banana ripening is mediated by enzymatic hydrolysis, and that ethylene signaling component MaEIL2 positively modulates starch breakdown via trans-activation of MaAMY3, MaISA2 and MaISA3.



中文翻译:

由乙烯信号组分 MaEIL2 介导的乙烯诱导的香蕉淀粉降解

淀粉是自然界中主要的储备碳水化合物,具有营养价值和工业应用价值。淀粉降解是香蕉果实软化和变甜的重要因素,但该过程的更多细节尚未完全了解。在本研究中,香蕉果实成熟过程中总淀粉、直链淀粉和支链淀粉的含量逐渐降低,这与总可溶性糖含量的升高是平行的。特别是,随着成熟的进行,观察到淀粉颗粒的尺寸减小和形状拉长,以及结晶度降低,这主要是由于淀粉酶和异淀粉酶等淀粉降解酶的消化。重要的是,乙烯信号组分 MaEIL2 与淀粉酶和异淀粉酶编码基因MaAMY3的启动子结合MaISA2MaISA3,并刺激它们的转录。总的来说,这些发现表明香蕉成熟过程中的淀粉糖转化是由酶水解介导的,并且乙烯信号成分 MaEIL2 通过MaAMY3MaISA2MaISA3 的反式激活积极调节淀粉分解。

更新日期:2021-07-13
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