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Adenosine Triggers Larval Settlement and Metamorphosis in the Mussel Mytilopsis sallei through the ADK-AMPK-FoxO Pathway
ACS Chemical Biology ( IF 4 ) Pub Date : 2021-07-13 , DOI: 10.1021/acschembio.1c00175
Jian He 1, 2 , Zhiwen Wu 1 , Liying Chen 1 , Qi Dai 1 , Huanhuan Hao 1 , Pei Su 1 , Caihuan Ke 1, 3 , Danqing Feng 1
Affiliation  

Settlement and metamorphosis of planktonic larvae into benthic adults are critical components of a diverse range of marine invertebrate-mediated processes such as the formation of mussel beds and coral reefs, the recruitment of marine shellfisheries, and the initiation of macrobiofouling. Although larval settlement and metamorphosis induced by natural chemical cues is widespread among marine invertebrates, the mechanisms of action remain poorly understood. Here, we identified that the molecular target of adenosine (an inducer of larval settlement and metamorphosis from conspecific adults in the invasive biofouling mussel Mytilopsis sallei) is adenosine kinase (ADK). The results of transcriptomic analyses, pharmacological assays, temporal and spatial gene expression analyses, and siRNA interference, suggest that ATP-dependent phosphorylation of adenosine catalyzed by ADK activates the downstream AMPK-FoxO signaling pathway, inducing larval settlement and metamorphosis in M. sallei. This study not only reveals the role of the ADK-AMPK-FoxO pathway in larval settlement and metamorphosis of marine invertebrates but it also deepens our understanding of the functions and evolution of adenosine signaling, a process that is widespread in biology and important in medicine.

中文翻译:

腺苷通过 ADK-AMPK-FoxO 通路在贻贝中触发幼虫定居和变态

浮游幼虫向底栖成虫的沉降和变态是各种海洋无脊椎动物介导的过程的关键组成部分,例如贻贝床和珊瑚礁的形成、海洋贝类的补充以及大型生物污垢的开始。尽管由天然化学线索引起的幼虫沉降和变态在海洋无脊椎动物中很普遍,但其作用机制仍知之甚少。在这里,我们确定了腺苷(幼虫附着和变态的同种成人的诱导剂的分子靶标侵入生物附着贻贝沙筛贝) 是腺苷激酶 (ADK)。转录组学分析、药理学分析、时空基因表达分析和 siRNA 干扰的结果表明,由 ADK 催化的腺苷 ATP 依赖性磷酸化激活下游 AMPK-FoxO 信号通路,诱导M. salle幼虫沉降和变态。这项研究不仅揭示了 ADK-AMPK-FoxO 通路在海洋无脊椎动物幼虫定居和变态中的作用,而且还加深了我们对腺苷信号传导功能和进化的理解,这一过程在生物学中很普遍,在医学中很重要。
更新日期:2021-08-20
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