Chemical Data Collections Pub Date : 2021-07-12 , DOI: 10.1016/j.cdc.2021.100751 Jean Garba Koffi 1, 2 , Rodrigue Keumoe 3 , Cyrille Armel Njanpa Ngansop 3 , Donald Ulrich Kenou Kagho 1 , Billy Toussie Tchegnitegni 4 , Yannick Stéphane Fongang Fotsing 5 , Jean Jules Kezetas Bankeu 6 , Fabrice Fekam Boyom 3 , Norbert Sewald 7 , Bruno Ndjakou Lenta 2
The CH2Cl2–MeOH (1:1) extract of Adenia lobata (stem bark) and the MeOH extracts of Endodesmia calophylloides (stem bark and leaves) displayed in vitro antileishmanial activities during preliminary screening against Leishmania donovani promastigotes with IC50 values ranging from 17.75–24.33 µg/mL. The fractions obtained from these extracts were also assessed for their antileishmanial (IC50 values between 9.19 and 100 µg/mL) and cytotoxicity activities. The purification of the active fractions led to the isolation of 24 compounds: seven from A. lobata (1-7) including one new ceramide (1) and seventeen from E. calophylloides (8-24). Their structures were elucidated based on the analysis of their NMR and MS data. The isolates were assessed for their antileishmanial activity and for their cytotoxicity. Germanicol caffeoyl ester (3), the (1:1) mixture of isoxanthochymol (18)/cycloxanthochymol (19), and the (1:1) mixture of tachioside (10)/isotachioside (11) displayed potent antileishmanial activity with IC50 values of 6.03, 4.77, and 8.66 µg/mL, respectively, with SI˃1.
中文翻译:
Endodesmia calophylloides Benth的成分。和Adenia lobata (Jacq.) Engl。与反兴奋剂活动
在针对杜氏利什曼原虫前鞭毛体的初步筛选过程中,Adenia lobata(茎皮)的 CH 2 Cl 2 –MeOH (1:1) 提取物和Endodesmia calophylloides(茎皮和叶)的 MeOH 提取物显示出体外抗利什曼原虫活性,IC 50值范围为从 17.75–24.33 µ g/mL。还评估了从这些提取物中获得的级分的抗利什曼原虫(IC 50值介于 9.19 和 100 µg /mL 之间)和细胞毒性活性。活性组分的纯化导致分离出 24 种化合物:7 种来自A. lobata (1-7 ) 包括一种新的神经酰胺 ( 1 ) 和十七种来自E. calophylloides ( 8-24 )。基于对它们的NMR和MS数据的分析阐明了它们的结构。评估分离株的抗利什曼原虫活性和细胞毒性。日耳曼尼醇咖啡酰酯 ( 3 )、异黄草酚 ( 18 )/环黄草酚 ( 19 ) 的 (1:1) 混合物和刺槐糖苷 ( 10 )/异立草木苷 ( 11 )的 (1:1) 混合物显示出有效的抗蛇毒活性,IC 50 SI˃1 的值分别为 6.03、4.77和 8.66 µ g/mL。