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Prostaglandin EP4 receptor mRNA expression in canine lymphoma
Veterinary and Comparative Oncology ( IF 2.1 ) Pub Date : 2021-07-11 , DOI: 10.1111/vco.12753
Margaret L Musser 1 , Austin K Viall 2 , Rachel L Phillips 2 , Olufemi Fasina 2 , Chad M Johannes 1
Affiliation  

Canine lymphoma (LSA) is a diverse, aggressive malignancy initiated by a variety of factors. Understanding those factors could help identify potential treatment options. Chronic inflammation drives lymphoma in human medicine and is suspected to play a role in veterinary medicine. The exact mechanisms, however, have not been elucidated. Upregulation of the cyclooxygenase enzymes, and subsequently prostaglandins, potentially play a stimulatory role. Prostaglandins work through one of four EP receptors (EP1-EP4) and the effects mediated through EP4R specifically are thought to be the primary drivers of cancer development. In human T-cell LSA, overexpression of EP4R has been found and appears to protect LSA cells from apoptosis. The role of EP4R in human B-cell LSA is more nuanced. This study aims to evaluate the mRNA expression of the EP4R gene (ptger4) in canine B-cell and T-cell LSA. Archived canine lymph nodes with histologically confirmed B-cell and T-cell LSA, and reactive lymph nodes, were evaluated for EP4R mRNA expression using a novel RNA in situ hybridization technique (RNAscope). Quantification of RNAscope signals was completed with an advanced digital pathology image analysis system (HALO). Results were reported as copy number, H-score, and percent tumour cell expression of EP4R mRNA. All reactive, B-cell LSA, and T-cell LSA lymph nodes expressed EP4R mRNA. The mRNA copy number, H-score, and percent tumour cell expression of EP4R were higher in B-cell (p < .003) and T-cell (p < .001) LSA samples compared to reactive lymph node samples. There were no differences between B-cell LSA and T-cell LSA.

中文翻译:

犬淋巴瘤中前列腺素 EP4 受体 mRNA 的表达

犬淋巴瘤 (LSA) 是由多种因素引发的多种侵袭性恶性肿瘤。了解这些因素有助于确定潜在的治疗方案。慢性炎症在人类医学中驱动淋巴瘤,并被怀疑在兽医学中发挥作用。然而,确切的机制尚未阐明。环氧合酶和随后的前列腺素的上调可能起到刺激作用。前列腺素通过四种 EP 受体 (EP1-EP4) 中的一种起作用,通过 EP4R 介导的作用被认为是癌症发展的主要驱动因素。在人类 T 细胞 LSA 中,已发现 EP4R 的过表达并似乎保护 LSA 细胞免于凋亡。EP4R 在人类 B 细胞 LSA 中的作用更为细微。本研究旨在评估 EP4R 基因的 mRNA 表达(ptger4 ) 在犬 B 细胞和 T 细胞 LSA 中。使用一种新的 RNA 原位杂交技术 (RNAscope) 评估具有组织学证实的 B 细胞和 T 细胞 LSA 和反应性淋巴结的存档犬淋巴结的 EP4R mRNA 表达。使用先进的数字病理图像分析系统 (HALO) 完成了 RNAscope 信号的量化。结果报告为 EP4R mRNA 的拷贝数、H 分数和肿瘤细胞表达百分比。所有反应性 B 细胞 LSA 和 T 细胞 LSA 淋巴结均表达 EP4R mRNA。 与反应性淋巴结样本相比,B 细胞 ( p  < .003) 和 T 细胞 ( p < .001) LSA 样本中 EP4R 的 mRNA 拷贝数、H 评分和肿瘤细胞表达百分比更高。B 细胞 LSA 和 T 细胞 LSA 之间没有差异。
更新日期:2021-07-11
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