In this study, the effects of organic nitrogen supplements, such as tryptone and peptone, on microbial growth and ethanol and acetic acid production were investigated to improve ethanol productivity in a Clostridium autoethanogenum culture using syngas as the sole carbon source. The concentration of yeast extract, which is an organic nitrogen source in a basal medium, was optimized to 0.5 g/L before adding the supplements. In the C. autoethanogenum culture using tryptone and peptone as supplements, microbial growth and acetic acid formation were proportionate to the supplement concentration till up to 5.0 g/L of tryptone or peptone. However, ethanol concentration reached a maximum level of 4.57 g/L and 4.23 g/L in the cultures supplemented with 0.5 g/L of tryptone and 0.5 g/L of peptone, respectively. Ethanol production did not increase at a supplement concentration higher than 0.5 g/L. Thus, the addition of tryptone and peptone at a concentration of 0.5 g/L showed the best volumetric ethanol productivities (0.286 and 0.264 g/L·d, respectively), which were enhanced by 45.9% and 44.3% compared to the results of the control experiment.

在这项研究中，研究了有机氮补充剂（如胰蛋白胨和蛋白胨）对微生物生长以及乙醇和乙酸生产的影响，以提高使用合成气作为唯一碳源的自产乙醇梭菌培养物中的乙醇生产率。在添加补充剂之前，酵母提取物（基础培养基中的有机氮源）的浓度优化为 0.5 g/L。在C. autoethanogenum使用胰蛋白胨和蛋白胨作为补充物进行培养时，微生物生长和醋酸的形成与补充物浓度成正比，直至胰蛋白胨或蛋白胨达到 5.0 g/L。然而，乙醇浓度在分别补充了 0.5 g/L 胰蛋白胨和 0.5 g/L 蛋白胨的培养物中达到了 4.57 g/L 和 4.23 g/L 的最高水平。乙醇产量在高于 0.5 g/L 的补充浓度下没有增加。因此，添加浓度为 0.5 g/L 的胰蛋白胨和蛋白胨表现出最佳的体积乙醇生产率（分别为 0.286 和 0.264 g/L·d），与实验结果相比分别提高了 45.9% 和 44.3%。对照实验。