The stems of Nauclea officinalis have been utilized as a crude drug in China, so other parts of the plant are abandoned, resulting in a waste of traditional Chinese medicine resources. To determine the distribution and content of the alkaloids, phenolic acids and iridoid in different organs (stem, branch, leaf and bark) of this plant, a reliable method has been established using LC–MS/MS. Nine constituents, namely strictosamide, vincosamide, chlorogenic acid, sweroside, naucleamide B, protocatechuic acid, pumiloside, vanillic acid and cryptochlorogenic acid, were simultaneously determined in 6 min. Meanwhile, the antipyretic, anti-inflammatory and analgesic activities were evaluated for comparative analysis of the pharmacological activity of different parts of N. officinalis. The results showed that the content of active components in other organs of N. officinalis was higher than that in stems, and the pharmacological effects of branches and leaves were also better. The established approach could be helpful for the quality control of N. officinalis, and also provide necessary information for the rational utilization of resources.

中文翻译：

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铁皮不同部位的生物活性成分及药理活性比较分析

在我国，铁皮的茎被用作生药，其他部分被废弃，造成中药资源的浪费。为确定该植物不同器官（茎、枝、叶和树皮）中生物碱、酚酸和环烯醚萜的分布和含量，使用 LC-MS/MS 建立了可靠的方法。在6 min内同时测定了硬糖酰胺、长春花酰胺、绿原酸、獐牙菜苷、核苷B、原儿茶酸、普鲁米洛苷、香草酸和隐绿原酸九种成分。同时，评价其解热、抗炎、镇痛活性，对比分析药典不同部位的药理活性。. 结果表明，铁皮其他器官的活性成分含量高于茎，枝叶的药理作用也更好。所建立的方法有助于药用植物的质量控制，也为资源的合理利用提供必要的信息。