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Identification of reference genes provides functional insights into meiotic recombination suppressors in Gerbera hybrida
Horticultural Plant Journal ( IF 5.7 ) Pub Date : 2021-07-10 , DOI: 10.1016/j.hpj.2020.09.008
Fan Li 1 , Ying Cheng 2 , Lulin Ma 1 , Shenchong Li 1 , Jihua Wang 1
Affiliation  

Gerbera Hybrida is one of the important cut flowers across the world. The novel traits are the primarily market requirements and the breeding targets, mainly determined by the degree of genetic variation after hybridization. However, meiotic recombination is highly conserved in most eukaryotes which suppressed the crossover formation and limited the genetic diversity. Recently, several meiotic recombination suppressors have been identified and characterized in plants, whereas it remains elusive in G. hybrida. In order to characterize the expression patterns of these suppressors in G. hybrida, 20 candidate reference genes were identified from the transcriptome datasets of G. hybrida, and their expression stabilities during plant development were evaluated by geNorm, NormFinder and BestKeeper. Although the most stable reference genes were variable in different softwares, comprehensive ranking revealed that PGK2 was the most stable reference gene and GAPDH was the most unstable one. The expression patterns of FANCM, FIGL1, RECQ4, RM1, and FLIP further validated that PGK2 was suitable for normalization of gene expression. Our study identified a reliable reference gene for gene expression during meiotic recombination, and provided functional insights into meiotic recombination suppressors in G. hybrida.



中文翻译:

参考基因的鉴定提供了对非洲菊减数分裂重组抑制因子的功能见解

非洲菊是世界上重要的切花之一。新性状主要是市场需求和育种目标,主要由杂交后的遗传变异程度决定。然而,减数分裂重组在大多数真核生物中是高度保守的,这抑制了交叉形成并限制了遗传多样性。最近,已经在植物中鉴定并表征了几种减数分裂重组抑制因子,而在G. hybrida 中它仍然难以捉摸。为了表征这些抑制基因在G. hybrida 中的表达模式,从G. hybrida的转录组数据集中确定了 20 个候选参考基因, 并且它们在植物发育过程中的表达稳定性由 geNorm、NormFinder 和 BestKeeper 评估。虽然最稳定的内参基因在不同的软件中是可变的,但综合排名显示PGK2是最稳定的内参基因,GAPDH是最不稳定的。FANCM、FIGL1、RECQ4、RM1FLIP的表达模式进一步验证了PGK2适用于基因表达的标准化。我们的研究确定了减数分裂重组过程中基因表达的可靠参考基因,并为G. hybrida 中的减数分裂重组抑制因子提供了功能见解。

更新日期:2021-07-10
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