当前位置: X-MOL 学术J. Hortic. Sci. Biotechnol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Integration of Korean S haplotypes controlling self-incompatibility into the unified nomenclature in radish (Raphanus sativus L.)
The Journal of Horticultural Science and Biotechnology ( IF 1.9 ) Pub Date : 2021-07-10 , DOI: 10.1080/14620316.2021.1942240
Ganghee Cho 1 , Sunggil Kim 1
Affiliation  

ABSTRACT

Self-incompatibility in radish (Raphanus sativus L.) is controlled by the S haplotype consisting of three tightly linked genes: SRK, SCR/SP11, and SLG. Since many radish S haplotypes have been reported with different nomenclature by several research groups, such discrepancy has resulted in confusion. To integrate S haplotypes identified in previous studies of Korean radish breeding lines into a unified nomenclature system, the nucleotide sequences of SRK and SLG genes isolated by different research groups were compared with each other. Two radish accessions whose draft whole-genome sequences were previously reported were shown to harbour class II RsS-9 and class I RsS-19 haplotypes, respectively. Nucleotide sequences of SLG and S domains of SRK genes were obtained from diverse breeding lines using four degenerate and 13 haplotype-specific primer pairs. A total of 27 out of 31 S haplotypes isolated in our previous study showed that SRK and SLG sequences were almost identical to those reported by other research groups. In addition, eight novel putative SRK or SLG sequences showing high homology with other SRK genes were identified. Finally, 27 S haplotypes were successfully integrated into the unified nomenclature, and the four S haplotypes containing unique sequences were renamed following the unified nomenclature.



中文翻译:

将控制自交不亲和性的韩国 S 单倍型整合到萝卜的统一命名中 (Raphanus sativus L.)

摘要

萝卜 ( Raphanus sativus L.) 的自交不亲和性由 S 单倍型控制,该S单倍型由三个紧密连锁的基因组成:SRK、SCR/SP11SLG。由于几个研究小组已经报道了许多具有不同命名法的萝卜S单倍型,因此这种差异导致了混乱。为了将之前韩国萝卜育种系研究中确定的S单倍型整合到一个统一的命名系统中, SRKSLG的核苷酸序列将不同研究组分离的基因相互比较。先前报道了其全基因组序列草案的两个萝卜种质分别显示具有 II 类RsS-9和 I 类RsS-19单倍型。SRK基因的SLGS结构域的核苷酸序列是使用四个简并和 13 个单倍型特异性引物对从不同的育种系中获得的。在我们之前的研究中分离的 31 个S单倍型中总共有 27 个表明SRKSLG序列与其他研究组报告的序列几乎相同。此外,八种新的推定SRK鉴定出与其他SRK基因具有高度同源性的SLG序列。最终,27个S单倍型成功整合到统一命名法中,4个含有独特序列的S单倍型按照统一命名法重新命名。

更新日期:2021-07-10
down
wechat
bug