Understanding factors that affect the catalytic efficiency and synergism of enzymes is helpful to enhance the process of bioconversion. In this study, birch wood (BW) was sequentially treated by delignification (DL), deacetylation (DA), and decrystallization (DC) treatments. The physiochemical structures of treated BW were characterized. Moreover, the influences of sequential treatments on the catalytic efficiency and synergism of xylanase and cellulase were studied. DL treatments efficiently improved the conversion of cellulose and xylan. A high degree of synergy (DS) between xylanase and cellulase was produced during hydrolysis of DL-treated BW. DA treatments enhanced xylan conversion but reduced the DS between xylanase and cellulase for xylan hydrolysis, whereas DC treatments enhanced cellulose conversion but reduced the DS between xylanase and cellulase for cellulose hydrolysis. The cellulose conversion of lithium chloride/N,N-dimethylacetamide (LiCl/DMAc)-treated BW (89.69%) was higher than the cellulose conversion of ball milling (BM)-treated BW (81.63%), whereas the xylan conversion of LiCl/DMAc-treated BW (83.77%) was lower than the xylan conversion of BM-treated BW (87.21%). This study showed that the catalytic efficiency and synergism of xylanase and cellulase are markedly affected by lignin hindrance, hemicellulose acetylation, and cellulose crystallization.

Graphical abstract

中文翻译：

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白桦木酶解过程中木聚糖酶和纤维素酶催化效率及协同作用的影响因素

了解影响酶催化效率和协同作用的因素有助于增强生物转化过程。在这项研究中，桦木 (BW) 依次通过去木质 (DL)、去乙酰化 (DA) 和去结晶 (DC) 处理。表征了处理过的BW的理化结构。此外，还研究了序贯处理对木聚糖酶和纤维素酶的催化效率和协同作用的影响。DL 处理有效地提高了纤维素和木聚糖的转化率。在 DL 处理的 BW 的水解过程中，木聚糖酶和纤维素酶之间产生了高度的协同作用 (DS)。DA处理增强了木聚糖转化，但降低了木聚糖酶和纤维素酶之间用于木聚糖水解的DS，而 DC 处理增强了纤维素转化，但降低了木聚糖酶和纤维素酶之间用于纤维素水解的 DS。氯化锂/N,N-二甲基乙酰胺 (LiCl/DMAc) 处理的 BW 的纤维素转化率 (89.69%) 高于球磨 (BM) 处理的 BW 的纤维素转化率 (81.63%)，而 LiCl 的木聚糖转化率/DMAc 处理的 BW (83.77%) 低于 BM 处理的 BW (87.21%) 的木聚糖转化率。该研究表明，木聚糖酶和纤维素酶的催化效率和协同作用受到木质素阻碍、半纤维素乙酰化和纤维素结晶的显着影响。而 LiCl/DMAc 处理的 BW 的木聚糖转化率 (83.77%) 低于 BM 处理的 BW 的木聚糖转化率 (87.21%)。该研究表明，木聚糖酶和纤维素酶的催化效率和协同作用受到木质素阻碍、半纤维素乙酰化和纤维素结晶的显着影响。而 LiCl/DMAc 处理的 BW 的木聚糖转化率 (83.77%) 低于 BM 处理的 BW 的木聚糖转化率 (87.21%)。该研究表明，木聚糖酶和纤维素酶的催化效率和协同作用受到木质素阻碍、半纤维素乙酰化和纤维素结晶的显着影响。

图形概要