Regard to this fact that the main transmission route of HPV and HHV-8 is via sexual activity, it is reasonable to speculate that coinfection of HPV and HHV-8 may have been played an important role in the development of cervical cancer. The aim of this study was to estimate the prevalence of HHV-8 and the frequency of HPV and HHV-8 coinfection in cervical samples of patients with cervical cancer and healthy individuals. In total, 364 samples from 61 patients with cervical cancer, 124 women with premalignant lesions, and 179 healthy individuals were investigated by nested-PCR. The frequency of HHV-8 was found to be 22.9%, 17.7%, and 14.5% in cervical cancer, premalignant lesions, and normal specimens, respectively (P = 0.308). The overall prevalence of coinfection between HHV-8 and HPV was shown to be 16.2%. The HPV prevalence was higher in HHV-8 positive samples than HHV-8 negative specimens in all three studied groups and this difference was reached a statistically significant level (P = 0.002). However, no significant differences were found between HHV-8 positivity and HPV genotypes (P = 0.08). Our results showed the higher rate of HHV-8 genome detection in cervical cancer group than control group. However, future studies with larger sample sizes and evaluation of expression of HHV-8 proteins are warranted.

中文翻译：

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伊朗女性正常、癌前和恶性宫颈样本中人类疱疹病毒 8 的流行率

鉴于 HPV 和 HHV-8 的主要传播途径是通过性行为这一事实，推测 HPV 和 HHV-8 的共感染可能在宫颈癌的发展中发挥了重要作用是合理的。本研究的目的是估计宫颈癌患者和健康个体的宫颈样本中 HHV-8 的患病率以及 HPV 和 HHV-8 合并感染的频率。通过巢式 PCR 研究了来自 61 名宫颈癌患者、124 名患有癌前病变的女性和 179 名健康个体的 364 份样本。在宫颈癌、癌前病变和正常标本中发现 HHV-8 的频率分别为 22.9%、17.7% 和 14.5%（P = 0.308）。HHV-8 和 HPV 共感染的总体流行率为 16.2%。在所有三个研究组中，HHV-8 阳性样本中的 HPV 患病率均高于 HHV-8 阴性样本，并且这种差异达到了统计学显着水平（P = 0.002）。然而，在 HHV-8 阳性和 HPV 基因型之间没有发现显着差异（P = 0.08）。我们的结果显示宫颈癌组的HHV-8基因组检测率高于对照组。然而，未来需要进行更大样本量的研究和评估 HHV-8 蛋白的表达。我们的结果显示宫颈癌组的HHV-8基因组检测率高于对照组。然而，未来需要进行更大样本量的研究和评估 HHV-8 蛋白的表达。我们的结果显示宫颈癌组的HHV-8基因组检测率高于对照组。然而，未来需要进行更大样本量的研究和评估 HHV-8 蛋白的表达。