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Artificial Diets With Different Protein Levels for Rearing Spodoptera frugiperda (Lepidoptera: Noctuidae)
Journal of Insect Science ( IF 2.2 ) Pub Date : 2021-07-02 , DOI: 10.1093/jisesa/ieab041
Caio Cesar Truzi 1 , Natalia Fernanda Vieira 1 , Joice Mendonça de Souza 1 , Sergio Antonio De Bortoli 1
Affiliation  

Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae) is a pest of great economic importance which can feed on more than 300 plant species. As it is polyphagous, its host plants may have variable physical and chemical constitutions. This may influence larval development, as protein and carbohydrate levels are important factors for adequate biological development. The aim of this study was to evaluate insect developmental parameters as well as to compare the food consumption of S. frugiperda larvae reared using diets with different protein levels under laboratory conditions. Three artificial diet formulations were used: one typically used for routine laboratory rearing, based on bean, wheat germ and brewer’s yeast (D1); one containing half the original amount of protein (D2), and the other with twice the original amount of protein (D3). The relative consumption rate (RCR), relative growth rate (RGR), and efficiency of conversion of ingested food (ECI) for S. frugiperda fourth instar larvae varied among diets. The protein present in the diet influenced the duration of larval and pupal periods and pupal weight, but did not affect larval survival, fecundity and longevity of adults. The different protein levels in the diets did not negatively influence population growth, so these three diet variations can be used for mass rearing in the laboratory. However, the influence of these diets on successive generations of the insect remains untested.

中文翻译:

养殖草地夜蛾(鳞翅目:夜蛾科)的不同蛋白质水平的人工饲料

Spodoptera frugiperda (JE Smith, 1797) (Lepidoptera: Noctuidae) 是一种具有重要经济意义的害虫,可以以 300 多种植物为食。由于它是多食性的,它的寄主植物可能具有不同的物理和化学组成。这可能会影响幼虫的发育,因为蛋白质和碳水化合物水平是充分生物发育的重要因素。本研究的目的是评估昆虫发育参数,并比较在实验室条件下使用不同蛋白质水平的饮食饲养的 S. frugiperda 幼虫的食物消耗。使用了三种人工饲料配方:一种通常用于实验室常规饲养,基于豆类、小麦胚芽和啤酒酵母 (D1);一种含有一半的蛋白质(D2),另一种含有两倍的蛋白质(D3)。S. frugiperda 四龄幼虫的相对消耗率 (RCR)、相对生长率 (RGR) 和摄取食物转化效率 (ECI) 因饮食而异。饮食中存在的蛋白质影响幼虫和蛹期的持续时间以及蛹的重量,但不影响幼虫的存活率、成虫的繁殖力和寿命。饮食中不同的蛋白质水平不会对种群增长产生负面影响,因此这三种饮食变化可用于实验室的大规模饲养。然而,这些饮食对昆虫后代的影响仍未得到检验。饮食中存在的蛋白质影响幼虫和蛹期的持续时间以及蛹的重量,但不影响幼虫的存活率、成虫的繁殖力和寿命。饮食中不同的蛋白质水平不会对种群增长产生负面影响,因此这三种饮食变化可用于实验室的大规模饲养。然而,这些饮食对昆虫后代的影响仍未得到检验。饮食中存在的蛋白质影响幼虫和蛹期的持续时间以及蛹的重量,但不影响幼虫的存活率、成虫的繁殖力和寿命。饮食中不同的蛋白质水平不会对种群增长产生负面影响,因此这三种饮食变化可用于实验室的大规模饲养。然而,这些饮食对昆虫后代的影响仍未得到检验。
更新日期:2021-07-02
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