Red sea bream (Pagrus major) is described as one of the marine fishes with high economic value, which is widely cultivated in East Asia. With large-scale and intensive aquaculture, bacterial diseases outbreak more frequently than before. In order to effectively prevent and control Vibrio anguillarum disease in red sea bream, we systematically analyzed the immune response mechanism of the head kidney and spleen of P. major infected with Vibrio anguillarum by RNAseq technology. In this study, we obtained 225,106,036 clean reads. By using Nr, Nt, Pfam, KOG/COG, Swiss-prot, KEGG, GO databases, we annotated 85,595 genes. In the head kidney and spleen, we obtained 3963 and 4981 DEGs (different expressed genes) respectively. To further study the function of DEGs, we performed GO and KEGG enrichment analysis on them. In pathway enrichment, the pathways which significantly enriched in both head kidney and spleen included Antigen processing and presentation, Phagocytosis and Cell adhesion molecules. In the head kidney, Ubiquitin-mediated proteolysis was specially enriched; while Intestinal immune network for IgM production, Complement and coagulation cascade were specially enriched in the spleen. This study provide effective data support for the comprehensive analysis of mechanism in P. major immune response following V. anguillarum infection, which would lay the foundation for further disease prevention and treatment.

红鲷鱼（Pagrus major）被描述为具有较高经济价值的海洋鱼类之一，在东亚广泛养殖。随着水产养殖规模化、集约化的发展，细菌性病害的爆发比以往更加频繁。为了有效预防和控制鳗弧菌在真鲷的疾病，我们系统地分析了头肾和脾脏的免疫应答机制P.主要感染鳗弧菌通过 RNAseq 技术。在这项研究中，我们获得了 225,106,036 个干净的读取。通过使用 Nr、Nt、Pfam、KOG/COG、Swiss-prot、KEGG、GO 数据库，我们注释了 85,595 个基因。在头肾和脾脏中，我们分别获得了3963和4981个DEGs（不同表达的基因）。为了进一步研究 DEG 的功能，我们对它们进行了 GO 和 KEGG 富集分析。在通路富集方面，在头肾和脾脏中均显着富集的通路包括抗原加工和呈递、吞噬作用和细胞粘附分子。在头肾中，泛素介导的蛋白水解特别丰富；而用于 IgM 产生、补体和凝血级联反应的肠道免疫网络在脾脏中特别丰富。本研究为综合分析其中的机理提供了有效的数据支持。P. anguillarum感染后的主要免疫反应，这将为进一步的疾病预防和治疗奠定基础。