Barcoding technology has greatly improved the throughput of cells and genes detected in single-cell RNA sequencing (scRNA-seq) studies. Recently, increasing studies have paid more attention to the use of this technology to increase the throughput of samples, as it has greatly reduced the processing time, technical batch effects, and library preparation costs, and lowered the per-sample cost. In this review, the various DNA-based barcoding methods for sample multiplexing are focused on, specifically, on the four major barcoding strategies. A detailed comparison of the barcoding methods is also presented, focusing on aspects such as sample/cell throughput and gene detection, and guidelines for choosing the most appropriate barcoding technique according to the personalized requirements are developed. Finally, the critical applications of sample multiplexing and technical challenges in combinatorial labeling, barcoding in vivo, and multimodal tagging at the spatially resolved resolution, as well as, the future prospects of multiplexed scRNA-seq, for example, prioritizing and predicting the severity of coronavirus disease 2019 (COVID-19) in patients of different gender and age are highlighted.

中文翻译：

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在单细胞分辨率下同时对样品进行大规模转录组分析的多路复用方法

条形码技术极大地提高了单细胞 RNA 测序 (scRNA-seq) 研究中检测到的细胞和基因的通量。最近，越来越多的研究更加关注使用该技术来提高样品通量，因为它大大减少了处理时间、技术批次效应和文库制备成本，并降低了每个样品的成本。在这篇综述中，用于样本复用的各种基于 DNA 的条形码方法重点关注，特别是四种主要的条形码策略。还介绍了条形码方法的详细比较，重点是样品/细胞通量和基因检测等方面，并制定了根据个性化要求选择最合适的条形码技术的指南。最后，