Herein, we report a method of fabricating strong and thermosensitive double network (T-DN) poly(N-isopropyl acrylamide) (PNIPAM)-based hydrogels, i.e. rigid and brittle poly(2-acrylamido-2-methylpropanesulfonic acid sodium salt) (PNaAMPS) as the first and soft and ductile poly(N-isopropyl acrylamide-co-acrylamide) (P(NIPAM-co-AAm)) as the second interpenetrating each other. In particular, NIPAM was deliberately integrated into the double network as an adjustor of elastic modulus and hydrophilicity, besides thermosensitivity. Such double network construction strategy resulted in PNaAMPS/P(NIPAM-co-AAm) T-DN hydrogels of excellent mechanical properties (0.83–1.37 MPa) and desirable temperature-dependent swellabilities. Besides, T-DN hydrogels with various NIPAM contents exhibited good biocompatibility with high cell survival rates around normal body temperatures. Furthermore, crystal violet (CV) could be readily loaded to impart antibacterial functionality to the T-DN hydrogels against E. coli. The double network construction strategy could be adapted to fabricating high-strength antibacterial hydrogels for a broad range of biomedical applications.

中文翻译：

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具有抗菌功能的高强度热敏​​双网络水凝胶

在此，我们报告了一种制备强热敏双网络（T-DN）聚（N-异丙基丙烯酰胺）（PNIPAM）基水凝胶的方法，即刚性和脆性聚（2-丙烯酰胺-2-甲基丙磺酸钠盐）（ PNaAMPS）作为第一个，柔软且可延展的聚（N-异丙基丙烯酰胺-co-丙烯酰胺）（P（NIPAM- co- AAm））作为第二个相互渗透。特别是，除了热敏性之外，NIPAM 还被故意集成到双网络中作为弹性模量和亲水性的调节剂。这种双网构建策略导致了 PNaAMPS/P(NIPAM- co-AAm) T-DN 水凝胶具有优异的机械性能 (0.83–1.37 MPa) 和理想的温度相关膨胀性。此外，具有各种 NIPAM 含量的 T-DN 水凝胶在正常体温下表现出良好的生物相容性和高细胞存活率。此外，结晶紫 (CV) 可以很容易地加载以赋予 T-DN 水凝胶抗大肠杆菌的抗菌功能。双网络构建策略可适用于制造用于广泛生物医学应用的高强度抗菌水凝胶。